IHC (Immunohiostchemistry)
(Factor B was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Factor B Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
Rabbit Factor B Polyclonal Antibody | anti-CFB antibody
Anti-Factor B Antibody
Gene Names
CFB; BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14
Reactivity
HumanNo cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Factor B, Antibody; Anti-Factor B Antibody; AHUS4; B factor properdin; BF; BFD; C3 proaccelerator; C3 proactivator; CFAB; CFB; Complement Factor B; FB; FBI12; GBG; Glycine rich beta glycoprotein; H2 Bf; H2BF; PBF2; Properdin factor B; P00751; Complement factor B; anti-CFB antibody
Host
Rabbit
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
764
Applicable Applications for anti-CFB antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human Factor B recombinant protein (Position: E518-L764). Human Factor B shares 84.2% amino acid (aa) sequence identity with mouse Factor B.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Factor B was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Factor B Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Factor B was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Factor B Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
WB (Western Blot)
(Western blot analysis of Factor B expression in A549 whole cell lysates (lane 1), 293T whole cell lysates (lane 2) and HELA whole cell lysates (lane 3). Factor B at 86KD was detected using rabbit anti- Factor B Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
WB (Western Blot)
(Western blot analysis of Factor B expression in A549 whole cell lysates (lane 1), 293T whole cell lysates (lane 2) and HELA whole cell lysates (lane 3). Factor B at 86KD was detected using rabbit anti- Factor B Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Related Product Information for anti-CFB antibody
Rabbit IgG polyclonal antibody for Complement factor B (CFB) detection.
Background: Complement factor B is a protein that in humans is encoded by the CFB gene. This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.
Background: Complement factor B is a protein that in humans is encoded by the CFB gene. This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.
References
1. "Entrez Gene: CFB complement factor B".
2. Benkmann, H. G., Goedde, H. W., Agarwal, D. P., Flatz, G., Rahimi, A., Kaifie, R. S., Delbruck, H. Properdin factor B polymorphism in Afghanistan. Hum. Hered. 30: 39-43, 1980.
3. Campbell, R. D., Porter, R. R. Molecular cloning and characterization of the gene coding for human complement protein factor B.Proc. Nat. Acad. Sci. 80: 4464-4468, 1983.
2. Benkmann, H. G., Goedde, H. W., Agarwal, D. P., Flatz, G., Rahimi, A., Kaifie, R. S., Delbruck, H. Properdin factor B polymorphism in Afghanistan. Hum. Hered. 30: 39-43, 1980.
3. Campbell, R. D., Porter, R. R. Molecular cloning and characterization of the gene coding for human complement protein factor B.Proc. Nat. Acad. Sci. 80: 4464-4468, 1983.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,872 Da
NCBI Official Full Name
complement factor B preproprotein
NCBI Official Synonym Full Names
complement factor B
NCBI Official Symbol
CFB
NCBI Official Synonym Symbols
BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14
NCBI Protein Information
complement factor B
UniProt Protein Name
Complement factor B
UniProt Gene Name
CFB
UniProt Synonym Gene Names
BF; BFD; GBG
UniProt Entry Name
CFAB_HUMAN
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Product Notes
The CFB cfb (Catalog #AAA46610) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Factor B Antibody reacts with Human No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Factor B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CFB cfb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Factor B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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