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product-image-AAA290230_FCM11.jpg FCM/FACS (Flow Cytometry) (FADS2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Rabbit FADS2 Polyclonal Antibody | anti-FADS2 antibody

FADS2 Antibody (Center)

Gene Names
FADS2; D6D; DES6; TU13; FADSD6; LLCDL2; SLL0262
Reactivity
Human (Predicted Reactivity: Bovine, Xenopus)
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FADS2, Antibody; FADS2 Antibody (Center); Fatty acid desaturase 2; 11419-; Delta(6) fatty acid desaturase; D6D; Delta(6) desaturase; Delta-6 desaturase; FADS2; anti-FADS2 antibody
Ordering
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FADS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-122 amino acids from the Central region of human FADS2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
96-122
Sequence Length
413
Applicable Applications for anti-FADS2 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(FADS2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA290230_FCM11.jpg FCM/FACS (Flow Cytometry) (FADS2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(FADS2 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).)

product-image-AAA290230_WB13.jpg WB (Western Blot) (FADS2 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded H.brain section using FADS2 Antibody (Center). AAA290230 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

product-image-AAA290230_IHC15.jpg IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H.brain section using FADS2 Antibody (Center). AAA290230 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Related Product Information for anti-FADS2 antibody
The protein encoded by this gene is a member of the fatty
acid desaturase (FADS) gene family. Desaturase enzymes regulate
unsaturation of fatty acids through the introduction of double
bonds between defined carbons of the fatty acyl chain. FADS family
members are considered fusion products composed of an N-terminal
cytochrome b5-like domain and a C-terminal multiple
membrane-spanning desaturase portion, both of which are
characterized by conserved histidine motifs. This gene is clustered
with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is
thought to have arisen evolutionarily from gene duplication based
on its similar exon/intron organization.
Product Categories/Family for anti-FADS2 antibody
References
Molto-Puigmarti, C., et al. Am. J. Clin. Nutr. (2010) In press :
Lattka, E., et al. Curr. Opin. Lipidol. 21(1):64-69(2010)
Lattka, E., et al. J. Lipid Res. 51(1):182-191(2010)
Glaser, C., et al. Metab. Clin. Exp. (2009) In press :
Lattka, E., et al. J Nutrigenet Nutrigenomics 2(3):119-128(2009)
Tang, C., et al. J. Lipid Res. 44(4):686-695(2003)
Nara, T.Y., et al. Biochem. Biophys. Res. Commun. 296(1):111-117(2002)
Marquardt, A., et al. Genomics 66(2):175-183(2000)
Cho, H.P., et al. J. Biol. Chem. 274(1):471-477(1999)
Stohr, H., et al. Genome Res. 8(1):48-56(1998)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52259
NCBI Official Full Name
fatty acid desaturase 2 isoform 2
NCBI Official Synonym Full Names
fatty acid desaturase 2
NCBI Official Symbol
FADS2
NCBI Official Synonym Symbols
D6D; DES6; TU13; FADSD6; LLCDL2; SLL0262
NCBI Protein Information
fatty acid desaturase 2
UniProt Protein Name
Fatty acid desaturase 2
UniProt Gene Name
FADS2
UniProt Synonym Gene Names
D6D; Delta(6) desaturase; Delta-6 desaturase
UniProt Entry Name
FADS2_HUMAN

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Product Notes

The FADS2 fads2 (Catalog #AAA290230) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 96-122. The FADS2 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's FADS2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the FADS2 fads2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FADS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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