WB (Western Blot)
(Detection of human and mouse FAM96B by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, RKO, K-562, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FAM96B antibody (AAA213447 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse FAM96B Polyclonal Antibody | anti-FAM96B antibody
Rabbit anti-FAM96B Antibody, Affinity Purified
Gene Names
FAM96B; MIP18; CGI-128
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
FAM96B, Antibody; Rabbit anti-FAM96B Antibody, Affinity Purified; MIP18; CGI-128; MSS19-interacting protein of 18 kDa; Protein FAM96B; mitotic spindle-associated MMXD complex subunit MIP18; FAM96B; CIA2B; cytosolic iron-sulfur assembly component 2B; family with sequence similarity 96 member B; Mitotic spindle-associated MMXD complex subunit MIP18; anti-FAM96B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-FAM96B antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse FAM96B by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, RKO, K-562, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FAM96B antibody (AAA213447 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse FAM96B by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, RKO, K-562, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FAM96B antibody (AAA213447 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human FAM96B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FAM96B antibody (AAA213447 lot 1) used for IP at 1 ug per reaction. FAM96B was also immunoprecipitated by rabbit anti-FAM96B antibody For blotting immunoprecipitated FAM96B, AAA213447 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human FAM96B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FAM96B antibody (AAA213447 lot 1) used for IP at 1 ug per reaction. FAM96B was also immunoprecipitated by rabbit anti-FAM96B antibody For blotting immunoprecipitated FAM96B, AAA213447 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for anti-FAM96B antibody
FAM96B is a component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As a CIA complex component and in collaboration with CIAO1 and MMS19, FAM96B binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y3D0].
Product Categories/Family for anti-FAM96B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,663 Da
NCBI Official Full Name
mitotic spindle-associated MMXD complex subunit MIP18
NCBI Official Synonym Full Names
family with sequence similarity 96, member B
NCBI Official Symbol
FAM96B
NCBI Official Synonym Symbols
MIP18; CGI-128
NCBI Protein Information
mitotic spindle-associated MMXD complex subunit MIP18; MSS19-interacting protein of 18 kDa
UniProt Protein Name
Mitotic spindle-associated MMXD complex subunit MIP18
UniProt Gene Name
FAM96B
UniProt Synonym Gene Names
MIP18; CGI-128; HSPC118
UniProt Entry Name
MIP18_HUMAN
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Product Notes
The FAM96B fam96b (Catalog #AAA213447) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-FAM96B Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FAM96B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FAM96B fam96b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAM96B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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