Rabbit FAM98A Polyclonal Antibody | anti-FAM98A antibody

Anti-FAM98A Antibody, Rabbit Polyclonal

Cat. Num. AAA254816

• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: FAM98A, Antibody; Anti-FAM98A Antibody, Rabbit Polyclonal; FAM98A Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-FAM98A Antibody; anti-FAM98A antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human FAM98A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-FAM98A antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human FAM98A
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human FAM98A, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(FAM98A was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-FAM98A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-FAM98A rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size: 57 kDa)

WB (Western Blot)

(Anti-FAM98A rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa)

Related Product Information for anti-FAM98A antibody

FAM98A was reported to be a member of the recently identified NN-CH family and is related to the yeast outer kinetochore components NDC80 and NUF2. The NN-CH family is defined by possession of the divergent calponin homology domain, which contains a different N-terminal domain but a similar CH domain. Additionally, it contains 7 repeated amino acid sequences, which are predicted to form a circular arrangement. This family includes NDC80, NUF2, FAM98A-C, CCDC22, CCDC93, C14orf166, NDC80/HEC1, NUF2, IFT81, IFT57, and CLUAP1. C14orf166, a member of the NN-CH family, is upregulated in bladder cancer cells and tissues, and could promote the proliferation of bladder tumor cells by regulating the cell cycle. FAM98A forms a molecular complex with PLHKEM1, DEF8, and NDEL1 that regulates lysosome positioning and secretion through RAB7. Moreover, FAM98A is arginine-methylated by protein arginine methyltransferase 1 (PRMT1) and is essential for malignancy of ovarian cancer cells. The kinetochore is composed of many conserved protein complexes that guide its specification, assembly, attachment to spindle microtubules, and regulation of chromosome segregation. Furthermore, the kinetochore provides an essential site of attachment for spindle microtubules during mitosis. Additionally, it controls a cell cycle checkpoint. As a microtubule-binding protein localized to centromeres, NDC80 acts on the checkpoint and is responsible for connection to non-stationary microtubules by polymerization and disaggregation. FAM98A is a novel component of stress granules. FAM98A is localized to stress granules, but not to P-bodies, after various stress stimuli. FAM98A associates with stress granule-localized proteins, such as DDX1, ATXN2, ATXN2L, and NUFIP2. Our results show a partial role of FAM98A for the organization of stress granules. Aberrant overexpression of FAM98A was found in endometrial carcinoma both in vitro and in vivo. FAM98A plays oncogenic roles in endometrial carcinoma. FAM98A is expressed in ovarian and colorectal cancer tissues.

References

Zheng R, et al. (2018) Fam98a promotes proliferation of non-small cell lung cancer cells via the p38-atf2 signaling pathway. Cancer Manag Res 10 2269-2278.Li Z, et al. (2019) Fam98a promotes cancer progression in endometrial carcinoma. Mol Cell Biochem 459 (1-2): 131-139.

NCBI and Uniprot Product Information

• NCBI GI #: 56699482
• NCBI GeneID: 25940
• NCBI Accession #: NP_056290.3
• NCBI GenBank Nucleotide #: NM_015475.3
• UniProt Accession #: Q8NCA5; Q9Y3Y6; B2RNA2
• Molecular Weight: 55,401 Da
• NCBI Official Full Name: protein FAM98A
• NCBI Official Synonym Full Names: family with sequence similarity 98, member A
• NCBI Official Symbol: FAM98A
• NCBI Protein Information: protein FAM98A
• UniProt Protein Name: Protein FAM98A
• UniProt Gene Name: FAM98A
• UniProt Entry Name: FA98A_HUMAN