IP (Immunoprecipitation)
(FAM98B was immunoprecipitated using:Lane A:0.5 mg Caco2 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-FAM98B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-FAM98B rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 37 kDaObserved band size :45 kDa)
Rabbit anti-Human FAM98B Polyclonal Antibody | anti-FAM98B antibody
Anti-FAM98B Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
FAM98B, Antibody; Anti-FAM98B Antibody, Rabbit Polyclonal; FAM98B Antibody, Rabbit PAb, Antigen Affinity Purified; anti-FAM98B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human FAM98B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-FAM98B antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the center region of the Human FAM98B
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human FAM98B, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(FAM98B was immunoprecipitated using:Lane A:0.5 mg Caco2 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-FAM98B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-FAM98B rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 37 kDaObserved band size :45 kDa)
IP (Immunoprecipitation)
(FAM98B was immunoprecipitated using:Lane A:0.5 mg Caco2 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-FAM98B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-FAM98B rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 37 kDaObserved band size :45 kDa)
WB (Western Blot)
(Anti-FAM98B rabbit polyclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLane B: Caco2 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:45 kDa)
WB (Western Blot)
(Anti-FAM98B rabbit polyclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLane B: Caco2 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:45 kDa)
Related Product Information for anti-FAM98B antibody
FAM98B associates with CLE (C14ORF166), DDX1, and HSPC117 (RTCB) in a ribonucleoprotein complex that shuttles RNA between the nucleus and cytosol in a transcription-dependent manner. FAM98B positively stimulates PRMT1-induced protein arginine dimethylated arginine methylation. FAM98B promotes colorectal cancer cell malignancy. FAM98A and FAM98B are working in the same axis as knockdown of both proteins together does not cause additional reduction in the cellular proliferation and colony formation of colorectal cancer cells.
References
Akter KA, et al. (2017) Fam98a associates with ddx1-c14orf166-fam98b in a novel complex involved in colorectal cancer progression. Int J Biochem Cell Biol 84 1-13.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,547 Da
NCBI Official Synonym Full Names
family with sequence similarity 98, member B
NCBI Official Symbol
FAM98B
NCBI Protein Information
protein FAM98B
UniProt Protein Name
Protein FAM98B
UniProt Gene Name
FAM98B
UniProt Entry Name
FA98B_HUMAN
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Product Notes
The FAM98B fam98b (Catalog #AAA254885) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FAM98B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAM98B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FAM98B fam98b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAM98B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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