Rabbit FAP Alpha Polyclonal Antibody | anti-FAP antibody

Rabbit Polyclonal to Human FAP Alpha

Cat. Num. AAA12367

• Gene Names: FAP; FAPA; SIMP; DPPIV
• Reactivity: Horse, Human, Monkey; Predicted Reactivity: Bovine, Pig
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: FAP Alpha, Antibody; Rabbit Polyclonal to Human FAP Alpha; Anti-FAP Alpha Antibody (Extracellular Domain) IHC-plus; DPPIV; FAP alpha; Fibroblast activation protein; FAPA; Seprase; Human FAP Alpha; anti-FAP antibody

• Host: Rabbit
• Reactivity: Horse, Human, Monkey; Predicted Reactivity: Bovine, Pig
• Clonality: Polyclonal
• Specificity: Human FAP. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 760

• Applicable Applications for anti-FAP antibody: Immunohistochemistry-Paraffin (IHC-p)
• Application Notes: IHC (5 ug/ml); (Optimal dilution to be determined by the researcher.)
• Target: FAP Alpha
• Immunogen: FAP Alpha antibody was raised against synthetic 17 amino acid peptide from extracellular domain of human FAP. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Horse (100%); Panda, Bovine, Pig (94%); Elephant, Dog (88%); Rat, Bat, Platypus (82%).
• Immunogen Type: Synthetic peptide
• Immunogen Species: FAP Alpha was raised against Human
• Family/Subfamily: Protease / Serine S9
• Epitope: Extracellular Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

IHC (Immunohistochemistry)

(Anti-FAP antibody IHC of human skin, hair follicle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-FAP antibody

Fibroblast Activation Protein Alpha (FAP) is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. FAP is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.

Product Categories/Family for anti-FAP antibody

Family: Protease; Subfamily: Serine S9

NCBI and Uniprot Product Information

• NCBI GI #: 16933540
• NCBI GeneID: 2191
• NCBI Accession #: NP_004451.2
• NCBI GenBank Nucleotide #: NM_004460.3
• UniProt Accession #: Q12884; O00199; Q53TP5; Q86Z29; Q99998; Q9UID4
• NCBI Official Full Name: prolyl endopeptidase FAP isoform 1
• NCBI Official Synonym Full Names: fibroblast activation protein, alpha
• NCBI Official Symbol: FAP
• NCBI Official Synonym Symbols: FAPA; SIMP; DPPIV
• NCBI Protein Information: prolyl endopeptidase FAP; seprase; FAPalpha; dipeptidyl peptidase FAP; surface-expressed protease; post-proline cleaving enzyme; gelatine degradation protease FAP; integral membrane serine protease; serine integral membrane protease; 170 kDa melanoma membrane-bound gelatinase
• UniProt Protein Name: Prolyl endopeptidase FAP
• UniProt Gene Name: FAP
• UniProt Entry Name: SEPR_HUMAN