IF (Immunofluorescence)
(AAA321995 staining Hela cells by IF/ICC at 1:100 (red).)
Rabbit anti-Human, Mouse FAP 1 Polyclonal Antibody | anti-FAP antibody
FAP 1 Antibody
Gene Names
FAP; FAPA; SIMP; DPPIV; FAPalpha
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
FAP 1, Antibody; FAP 1 Antibody; anti-FAP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
FAP 1 antibody detects endogenous levels of total FAP 1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Rabbit IgG in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-FAP antibody
IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human FAP
Similarity
Belongs to the peptidase S9B family.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA321995 staining Hela cells by IF/ICC at 1:100 (red).)
IF (Immunofluorescence)
(AAA321995 staining Hela cells by IF/ICC at 1:100 (red).)
WB (Western Blot)
(Western blot analysis of FAP 1 expression in LOVO cell extract)
WB (Western Blot)
(Western blot analysis of FAP 1 expression in LOVO cell extract)
IF (Immunofluorescence)
(AAA321995 staining LOVO cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA321995 staining LOVO cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-FAP antibody
Description: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90 kDa
NCBI Official Full Name
prolyl endopeptidase FAP isoform 2
NCBI Official Synonym Full Names
fibroblast activation protein alpha
NCBI Official Symbol
FAP
NCBI Official Synonym Symbols
FAPA; SIMP; DPPIV; FAPalpha
NCBI Protein Information
prolyl endopeptidase FAP
UniProt Protein Name
Prolyl endopeptidase FAP
UniProt Gene Name
FAP
UniProt Synonym Gene Names
FAPalphaBy similarityManual assertion inferred from sequence similarity toiUniProtKB:P97321Â (SEPR_MOUSE); SIMP1 PublicationManual assertion based on opinion iniRef.2; Seprase1 PublicationManual assertion based on opinion iniRef.11; APCE1 PublicationManua
Similar Products
Product Notes
The FAP fap (Catalog #AAA321995) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAP 1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FAP 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the FAP fap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAP 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.