WB (Western Blot)
(Western blot analysis of extracts from MCF7, using FARS2 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit FARS2 Polyclonal Antibody | anti-FARS2 antibody
FARS2 Antibody
Gene Names
FARS2; FARS1; PheRS; SPG77; COXPD14; HSPC320; mtPheRS
Reactivity
Human, Mouse, RatPredicted: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
FARS2, Antibody; FARS2 Antibody; dJ236A3.1 (phenylalanine tRNA synthetase); dJ520B18.2 (FARS1 (phenylalanine tRNA synthetase)); FARS1; Fars2; HSPC320; Phenylalanine translase; Phenylalanine tRNA ligase 2, mitochondrial; Phenylalanine tRNA ligase; Phenylalanine tRNA synthetase 1 (mitochondrial); Phenylalanine tRNA synthetase 2 (mitochondrial); Phenylalanine--tRNA ligase; Phenylalanyl tRNA synthetase 2; Phenylalanyl-tRNA synthetase, mitochondrial; PheRS; SYFM_HUMAN; anti-FARS2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog
Predicted: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
FARS2 Antibody detects endogenous levels of FARS2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
451
Applicable Applications for anti-FARS2 antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human FARS2.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Mitochondrion
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using FARS2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using FARS2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using FARS2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using FARS2 Antibody. The lane on the left was treated with blocking peptide.)
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Related Product Information for anti-FARS2 antibody
Description: Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins.
Subunit Structure: Monomer.
Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family.
Subunit Structure: Monomer.
Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 48 kDa
Predicted: 53 kDa
Predicted: 53 kDa
NCBI Official Full Name
phenylalanine--tRNA ligase, mitochondrial
NCBI Official Synonym Full Names
phenylalanyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol
FARS2
NCBI Official Synonym Symbols
FARS1; PheRS; SPG77; COXPD14; HSPC320; mtPheRS
NCBI Protein Information
phenylalanine--tRNA ligase, mitochondrial
UniProt Protein Name
Phenylalanine--tRNA ligase, mitochondrial
UniProt Gene Name
FARS2
UniProt Synonym Gene Names
FARS1; PheRS
UniProt Entry Name
SYFM_HUMAN
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Product Notes
The FARS2 fars2 (Catalog #AAA328854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FARS2 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's FARS2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the FARS2 fars2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FARS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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