WB (Western Blot)
(Detection of human FASN by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FASN antibody AAA211421 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. FASN was also immunoprecipitated by rabbit anti-FAS antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
Rabbit anti-Human, Mouse FASN Polyclonal Antibody | anti-FASN antibody
Rabbit anti-FASN Antibody, Affinity Purified
Gene Names
FASN; FAS; OA-519; SDR27X1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
FASN, Antibody; Rabbit anti-FASN Antibody, Affinity Purified; FAS; OA-519; short chain dehydrogenase/reductase family 27X, member 1; Oleoyl-[acyl-carrier-protein] hydrolase; fatty acid synthase; 3-oxoacyl-[acyl-carrier-protein] reductase; [Acyl-carrier-protein] S-acetyltransferase; 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; SDR27X1; [Acyl-carrier-protein] S-malonyltransferase; Enoyl-[acyl-carrier-protein] reductase; Fatty acid synthase; anti-FASN antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-FASN antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine
Immunogen
Between 2461 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human FASN by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FASN antibody AAA211421 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. FASN was also immunoprecipitated by rabbit anti-FAS antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
WB (Western Blot)
(Detection of human FASN by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FASN antibody AAA211421 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. FASN was also immunoprecipitated by rabbit anti-FAS antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
IHC (Immunohistochemistry)
(Detection of human and mouse FASN by immunohistochemistry. Sample: FFPE section of human prostate carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-FASN (Cat. No. AAA211421) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human and mouse FASN by immunohistochemistry. Sample: FFPE section of human prostate carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-FASN (Cat. No. AAA211421) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-FASN antibody
Fatty acid synthase (FASN) is a multifunctional protein that is mainly involved in catalyzing the synthesis of long-chain fatty acids. FASN has recently been viewed as an important protein involved in carcinogenesis as it has been found to be constitutively active in subsets of carcinomas and is associated with a poor prognosis. The inhibition of FASN activity in cancer cells promotes apoptosis and thus may play a role in maintaining cancer cell survival.
Product Categories/Family for anti-FASN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
273,427 Da
NCBI Official Full Name
fatty acid synthase
NCBI Official Synonym Full Names
fatty acid synthase
NCBI Official Symbol
FASN
NCBI Official Synonym Symbols
FAS; OA-519; SDR27X1
NCBI Protein Information
fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1
UniProt Protein Name
Fatty acid synthase
UniProt Gene Name
FASN
UniProt Synonym Gene Names
FAS
UniProt Entry Name
FAS_HUMAN
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Product Notes
The FASN fasn (Catalog #AAA211421) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-FASN Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FASN can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FASN fasn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FASN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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