Rabbit anti-Mouse FAT10 Polyclonal Antibody | anti-UBD antibody

FAT10 Antibody (C-term)

Cat. Num. AAA285266

• Gene Names: UBD; FAT10; UBD-3; GABBR1
• Reactivity: Mouse
• Applications: Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: FAT10, Antibody; FAT10 Antibody (C-term); Ubiquitin D; Diubiquitin; Ubiquitin-like protein FAT10; UBD; FAT10; anti-UBD antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This FAT10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-153 amino acids from the C-terminal region of human FAT10.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 120-153
• Sequence Length: 165

• Applicable Applications for anti-UBD antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Ubiquitin-like protein modifier which can becovalently attached to target protein and subsequently leads to their degradation bythe 26S proteasome, in a NUB1-dependentmanner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediatedactivation of the central mediator of innateimmunity NF-kappa-B by promotingTNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required forTNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). Maybe involved in dendritic cell (DC) maturation,the process by which immature dendritic cellsdifferentiate into fully competentantigen-presenting cells that initiate T-cellresponses. Mediates mitotic non-disjunctionand chromosome instability, in long- term invitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomeswhen proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium andtubular cells during renal diseases such aspolycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN).
• Cellular Location: Nucleus. Cytoplasm Note=Accumulates in aggresomes under proteasome inhibition conditions
• Tissue Location: Constitutively expressed in mature dendritic cells and B-cells. Mostly expressed in the reticuloendothelial system (e.g. thymus, spleen), the gastrointestinal system, kidney, lung and prostate gland.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of FAT10 Antibody (C-term) (AAA285266) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohiostchemistry)

(FAT10 Antibody (C-term) (AAA285266) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FAT10 Antibody (C-term) (AAA285266) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(FAT10 Antibody (C-term) (AAA285266) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the hFAT10 antibody detected the hFAT10 protein (arrow).)

Product Categories/Family for anti-UBD antibody

Cell Biology; Immunology; Microbiology; Signal Transduction

References

Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Frank, B., et al. Int. J. Cancer (2010) In press :
Gong, P., et al. J. Am. Soc. Nephrol. 21(2):316-326(2010)
Castellanos-Rubio, A., et al. Hum. Immunol. 71(1):96-99(2010)
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :

NCBI and Uniprot Product Information

• NCBI GI #: 222352096
• NCBI GeneID: 10537
• NCBI Accession #: NP_006389.2
• NCBI GenBank Nucleotide #: NM_006398.3
• UniProt Accession #: O15205; Q5STL2; Q5SUK2; Q96EC7; B0UZT6
• NCBI Official Full Name: ubiquitin D
• NCBI Official Synonym Full Names: ubiquitin D
• NCBI Official Symbol: UBD
• NCBI Official Synonym Symbols: FAT10; UBD-3; GABBR1
• NCBI Protein Information: ubiquitin D
• UniProt Protein Name: Ubiquitin D
• UniProt Gene Name: UBD
• UniProt Synonym Gene Names: FAT10
• UniProt Entry Name: UBD_HUMAN