Rabbit FBXL2 Polyclonal Antibody | anti-FBXL2 antibody

FBXL2 Antibody (C-term)

Cat. Num. AAA288939

• Gene Names: FBXL2; FBL2; FBL3
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: FBXL2, Antibody; FBXL2 Antibody (C-term); F-box/LRR-repeat protein 2; F-box and leucine-rich repeat protein 2 {ECO:0000312|HGNC:HGNC:13598}; F-box protein FBL2/FBL3 {ECO:0000303|PubMed:10945468; ECO:0000312|EMBL:AAF045101}; FBXL2 HGNC:13598); anti-FBXL2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This FBXL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-395 amino acids from the C-terminal region of human FBXL2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 366-395
• Sequence Length: 355

• Applicable Applications for anti-FBXL2 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(FBXL2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(FBXL2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of FBXL2 Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane).FBXL2 (arrow) was detected using the purified Pab.)

Related Product Information for anti-FBXL2 antibody

FBXL2 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats.

References

Ye, J. PLoS Pathog. 3 (8), E108 (2007)
Ilyin, G.P., et al. Genomics 67(1):40-47(2000)
Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 284447314
• NCBI GeneID: 25827
• NCBI Accession #: NP_001165184.1; NP_036289.3
• NCBI GenBank Nucleotide #: NM_001171713.1
• UniProt Accession #: Q9UKC9; Q6IAN3; Q9NVQ8; Q9UK27; Q9UKA5; Q9Y3Y9; B4DQV0; E9PD06
• Molecular Weight: 47062
• NCBI Official Full Name: F-box/LRR-repeat protein 2 isoform 2
• NCBI Official Synonym Full Names: F-box and leucine-rich repeat protein 2
• NCBI Official Symbol: FBXL2
• NCBI Official Synonym Symbols: FBL2; FBL3
• NCBI Protein Information: F-box/LRR-repeat protein 2
• UniProt Protein Name: F-box/LRR-repeat protein 2
• UniProt Gene Name: FBXL2
• UniProt Entry Name: FBXL2_HUMAN