IHC (Immunohistochemisry)
(Immunofluorescent analysis of HeLa cells using AAA231002 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human FBXW5 Polyclonal Antibody | anti-FBXW5 antibody
FBXW5 Antibody
Gene Names
FBXW5; Fbw5
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
FBXW5, Antibody; FBXW5 Antibody; F-box/WD repeat-containing protein 5 (F-box and WD-40 domain-containing protein 5); FBXW5; FBW5; anti-FBXW5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
566
Applicable Applications for anti-FBXW5 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Species
Human
Immunogen
Recombinant Human F-box/WD repeat-containing protein 5 protein (265-494AA)
Conjugate
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-160328 / sc-160329
Immunogen Species
Homo sapiens (Human)
Research Area
Cell Biology
Target Names
FBXW5
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
IHC (Immunohistochemisry)
(Immunofluorescent analysis of HeLa cells using AAA231002 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunofluorescent analysis of HeLa cells using AAA231002 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human appendix tissue using AAA231002 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human appendix tissue using AAA231002 at dilution of 1:100)
WB (Western Blot)
(Western blotAll lanes :FBXW5 antibody at 2 ug/mlLane 1 : PC-3 whole cell lysateLane 2 : Jurkat whole cell lysateLane 3 : Hela whole cell lysateLane 4 : 293T whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 64,43 kDaObserved band size: 64,41 kDa)
WB (Western Blot)
(Western blotAll lanes :FBXW5 antibody at 2 ug/mlLane 1 : PC-3 whole cell lysateLane 2 : Jurkat whole cell lysateLane 3 : Hela whole cell lysateLane 4 : 293T whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 64,43 kDaObserved band size: 64,41 kDa)
Related Product Information for anti-FBXW5 antibody
Substrate recognition component of both SCF (SKP1-CUL1-F-box protein) and DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Substrate recognition component of the SCF(FBXW5) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of SASS6 during S phase, leading to prevent centriole reduplication. The SCF(FBXW5) complex also mediates ubiquitination and degradation of actin-regulator EPS8 during G2 phase, leading to the transient degradation of EPS8 and subsequent cell shape changes required to allow mitotic progression. Substrate-specific adapter of the DCX(FBXW5) E3 ubiquitin-protein ligase complex which mediates the polyubiquitination and subsequent degradation of TSC2. May also act as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B (IL1B) signaling pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,185 Da
NCBI Official Full Name
F-box/WD repeat-containing protein 5
NCBI Official Synonym Full Names
F-box and WD repeat domain containing 5
NCBI Official Symbol
FBXW5
NCBI Official Synonym Symbols
Fbw5
NCBI Protein Information
F-box/WD repeat-containing protein 5
UniProt Protein Name
F-box/WD repeat-containing protein 5
UniProt Gene Name
FBXW5
UniProt Synonym Gene Names
FBW5
UniProt Entry Name
FBXW5_HUMAN
Similar Products
Product Notes
The FBXW5 fbxw5 (Catalog #AAA231002) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FBXW5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FBXW5 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the FBXW5 fbxw5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FBXW5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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