WB (Western Blot)
(Western blot analysis of lysate from human spleen tissue lysate, using FCER1G Antibody (C-term). AAA284806 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Rabbit FCER1G Polyclonal Antibody | anti-FCER1G antibody
FCER1G Antibody (C-term)
Gene Names
FCER1G; FCRG
Reactivity
HumanPredicted Reactivity: Bovine, Monkey, Mouse, Pig, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FCER1G, Antibody; FCER1G Antibody (C-term); High affinity immunoglobulin epsilon receptor subunit gamma; Fc receptor gamma-chain; FcRgamma; Fc-epsilon RI-gamma; IgE Fc receptor subunit gamma; FceRI gamma; FCER1G; anti-FCER1G antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Bovine, Monkey, Mouse, Pig, Rat
Predicted Reactivity: Bovine, Monkey, Mouse, Pig, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This FCER1G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-86 amino acids from the C-terminal region of human FCER1G.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-FCER1G antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Function
Associates with a variety of FcR alpha chains to form a functional signaling complex. Regulates several aspects of the immune response. The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface. Also involved in collagen-mediated platelet activation and in neutrophil activation mediated by integrin.
Cellular Location
Cell membrane; Single-pass type I membrane protein
Antigen Region
60-86
Function
Adapter protein containing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. As a component of the high-affinity immunoglobulin E (IgE) receptor, mediates allergic inflammatory signaling in mast cells. As a constitutive component ofinterleukin-3 receptor complex, selectively mediates interleukin 4/IL4 production by basophils, priming T-cells toward effector T-helper 2 subset. Associates with pattern recognition receptors CLEC4D and CLEC4Eto form a functional signaling complex in myeloid cells. Binding of mycobacterial trehalose 6,6'- dimycolate (TDM) to this receptor complex leads to phosphorylation of ITAM, triggering activation of SYK, CARD9and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen- specific priming of T-cells toward effector T-helper 1 and T-helper 17cell subtypes. May function cooperatively with other activating receptors. Functionally linked to integrin beta-2/ITGB2-mediatedneutrophil activation. Also involved in integrin alpha-2/ITGA2-mediated platelet activation.
Preparation and Storage
Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysate from human spleen tissue lysate, using FCER1G Antibody (C-term). AAA284806 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from human spleen tissue lysate, using FCER1G Antibody (C-term). AAA284806 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from human spleen tissue lysate, using FCER1G Antibody (C-term). AAA284806 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from human spleen tissue lysate, using FCER1G Antibody (C-term). AAA284806 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. spleen section using FCER1G Antibody (C-term). AAA284806 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. spleen section using FCER1G Antibody (C-term). AAA284806 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Related Product Information for anti-FCER1G antibody
The high affinity IgE receptor is a key molecule involved in allergic reactions. It is a tetramer composed of 1 alpha, 1 beta, and 2 gamma chains. The gamma chains are also subunits of other Fc receptors
Product Categories/Family for anti-FCER1G antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Untersmayr, E., et al. PLoS ONE 5 (2), E9023 (2010) :
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Untersmayr, E., et al. PLoS ONE 5 (2), E9023 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
high affinity immunoglobulin epsilon receptor subunit gamma
NCBI Official Synonym Full Names
Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
NCBI Official Symbol
FCER1G
NCBI Official Synonym Symbols
FCRG
NCBI Protein Information
high affinity immunoglobulin epsilon receptor subunit gamma
UniProt Protein Name
High affinity immunoglobulin epsilon receptor subunit gamma
UniProt Gene Name
FCER1G
UniProt Synonym Gene Names
FcRgamma; FceRI gamma
UniProt Entry Name
FCERG_HUMAN
Similar Products
Product Notes
The FCER1G fcer1g (Catalog #AAA284806) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FCER1G Antibody (C-term) reacts with Human Predicted Reactivity: Bovine, Monkey, Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FCER1G can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the FCER1G fcer1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FCER1G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.