WB (Western Blot)
(Western blot analysis of FDX1 using HepG2 whole lysates.)
Rabbit anti-Human FDX1 Polyclonal Antibody | anti-FDX1 antibody
FDX1 Antibody
Gene Names
FDX1; ADX; FDX; LOH11CR1D
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
FDX1, Antibody; FDX1 Antibody; Adrenal ferredoxin; Adrenodoxin; Adrenodoxin, mitochondrial; ADX; ADX_HUMAN; FDX; fdx1; Ferredoxin 1; Ferredoxin-1; Hepatoredoxin; LOH11CR1D; Mitochondrial adrenodoxin; mitochondrial; anti-FDX1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
FDX1 antibody detects endogenous levels of total FDX1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
184
Applicable Applications for anti-FDX1 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human FDX1
Subcellular Location
Mitochondrion Matrix.
Tissue Specificity
Highest levels in the adrenal gland (at protein level). Also detected in kidney and testis (at protein level).
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of FDX1 using HepG2 whole lysates.)
WB (Western Blot)
(Western blot analysis of FDX1 using HepG2 whole lysates.)
IF (Immunofluorescence)
(AAA327363 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA327363 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-FDX1 antibody
Function: Essential for the synthesis of various steroid hormones (PubMed:20547883, PubMed:21636783). Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis (PubMed:20547883, PubMed:21636783). Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage (PubMed:20547883, PubMed:21636783). Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons (By similarity).
Subunit Structure: Interacts with CYP11A1.
Similarity: Belongs to the adrenodoxin/putidaredoxin family.
Subunit Structure: Interacts with CYP11A1.
Similarity: Belongs to the adrenodoxin/putidaredoxin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 19 kDa
Predicted: 20 kDa
Predicted: 20 kDa
NCBI Official Full Name
adrenodoxin, mitochondrial
NCBI Official Synonym Full Names
ferredoxin 1
NCBI Official Symbol
FDX1
NCBI Official Synonym Symbols
ADX; FDX; LOH11CR1D
NCBI Protein Information
adrenodoxin, mitochondrial
UniProt Protein Name
Adrenodoxin, mitochondrial
UniProt Gene Name
FDX1
UniProt Synonym Gene Names
ADX
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Product Notes
The FDX1 fdx1 (Catalog #AAA327363) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FDX1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FDX1 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the FDX1 fdx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FDX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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