Rabbit anti-Human Ferritin Heavy Chain / FTH1 Polyclonal Antibody | anti-FTH1 antibody

Rabbit Polyclonal to Human Ferritin Heavy Chain / FTH1

Cat. Num. AAA52393

• Gene Names: FTH1; FHC; FTH; HFE5; PLIF; FTHL6; PIG15
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
• Purity: Protein A purified
• Synonyms: Ferritin Heavy Chain / FTH1, Antibody; Rabbit Polyclonal to Human Ferritin Heavy Chain / FTH1; Anti-Ferritin Heavy Chain / FTH1 Antibody (aa154-183) IHC-plus; FTH1; Apoferritin; Ferritin H subunit; Ferritin heavy chain; Ferritin; heavy polypeptide 1; FHC; FTHL6; PIG15; PLIF; FTH; Human Ferritin Heavy Chain; anti-FTH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This FTH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the C-terminal region of human FTH1.
• Purity/Purification: Protein A purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 154-183

• Applicable Applications for anti-FTH1 antibody: FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry)
• Target: FTH1 / Ferritin Heavy chain
• Immunogen Species: FTH1 / Ferritin Heavy chain antibody was raised against Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

WB (Western Blot)

(Western blot of FTH1 Antibody (C-term) in A2058 cell line lysates (35 ug/lane). FTH1 (arrow) was detected using the purified Pab.)

FCM/FACS (Flow Cytometry)

(Flow cytometry of HepG2 cells using FTH1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry)

(Anti-Ferritin Heavy Chain / FTH1 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

NCBI and Uniprot Product Information

• NCBI GI #: 56682959
• NCBI GeneID: 2495
• NCBI Accession #: NP_002023.2
• NCBI GenBank Nucleotide #: NM_002032.2
• UniProt Accession #: P02794; Q3KRA8; Q3SWW1; B3KNR5
• NCBI Official Full Name: ferritin heavy chain
• NCBI Official Synonym Full Names: ferritin, heavy polypeptide 1
• NCBI Official Symbol: FTH1
• NCBI Official Synonym Symbols: FHC; FTH; HFE5; PLIF; FTHL6; PIG15
• NCBI Protein Information: ferritin heavy chain; apoferritin; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein
• UniProt Protein Name: Ferritin heavy chain
• UniProt Gene Name: FTH1
• UniProt Synonym Gene Names: FTH; FTHL6; Ferritin H subunit
• UniProt Entry Name: FRIH_HUMAN