IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FGA Antibody (N-term) with HepG2 cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human FGA Polyclonal Antibody | anti-FGA antibody
FGA Antibody (N-term)
Gene Names
FGA; Fib2
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FGA, Antibody; FGA Antibody (N-term); Fibrinogen alpha chain; Fibrinopeptide A; FGA; anti-FGA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the N-terminal region of human FGA.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
116-144
Sequence Length
644
Applicable Applications for anti-FGA antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Function
Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re- epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
Cellular Location
Secreted
Tissue Location
Detected in blood plasma (at protein level).
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FGA Antibody (N-term) with HepG2 cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FGA Antibody (N-term) with HepG2 cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(FGA Antibody (N-term) (RB18707) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FGA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(FGA Antibody (N-term) (RB18707) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FGA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(FGA Antibody (N-term) western blot analysis in human blood plasma tissue lysates (35ug/lane).This demonstrates the FGA antibody detected the FGA protein (arrow).)
WB (Western Blot)
(FGA Antibody (N-term) western blot analysis in human blood plasma tissue lysates (35ug/lane).This demonstrates the FGA antibody detected the FGA protein (arrow).)
Related Product Information for anti-FGA antibody
FGA is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types.
Product Categories/Family for anti-FGA antibody
References
Thomas,V.A.,et.al., Biochemistry 37 (8), 2291-2298 (1998)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
94973
NCBI Official Full Name
fibrinogen alpha chain isoform alpha-E preproprotein
NCBI Official Synonym Full Names
fibrinogen alpha chain
NCBI Official Symbol
FGA
NCBI Official Synonym Symbols
Fib2
NCBI Protein Information
fibrinogen alpha chain
UniProt Protein Name
Fibrinogen alpha chain
UniProt Gene Name
FGA
UniProt Entry Name
FIBA_HUMAN
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Product Notes
The FGA fga (Catalog #AAA287837) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 116-144. The FGA Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FGA can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the FGA fga for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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