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product-image-AAA199032_WB8.jpg WB (Western Blot) (WB Suggested Anti-FGF2 Antibody Titration: 2 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate)

Rabbit FGF2 Polyclonal Antibody | anti-FGF2 antibody

FGF2 antibody - middle region

Gene Names
FGF2; BFGF; FGFB; FGF-2; HBGF-2
Reactivity
Tested: Human, Mouse
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
FGF2, Antibody; FGF2 antibody - middle region; anti-FGF2 antibody
Ordering
Host
Rabbit
Reactivity
Tested: Human, Mouse
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5mg/ml (varies by lot)
Sequence
Synthetic peptide located within the following region: RLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Sequence Length
288
Applicable Applications for anti-FGF2 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Predicted Homology Based on Immunogen Sequence
Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 93%; Zebrafish: 86%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human FGF2
Protein Size (#AA)
288 Amino Acids
Protein Interactions
TUBG1; HMT1; PRMT1; CRYAB; THBS1; UBC; API5; CEP57; FGFRL1; FGFBP1; CXCL13; RPL6; RPS19; PTX3; NRP1; GPC4; RPS6KA3; GPC3; VTN; SDC3; SDC1; PF4; HSPG2; SDC2; FGFR1; FGFR4; FGFR3; FGF2; CSNK2A2; CSNK2B; CSNK2A1; CD44; SDC4;
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(WB Suggested Anti-FGF2 Antibody Titration: 2 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate)

product-image-AAA199032_WB8.jpg WB (Western Blot) (WB Suggested Anti-FGF2 Antibody Titration: 2 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate)

WB (Western Blot)

product-image-AAA199032_WB10.jpg WB (Western Blot)

WB (Western Blot)

product-image-AAA199032_WB11.jpg WB (Western Blot)

IHC (Immunohiostchemistry)

(Sample Type: Human A375 cellsPrimary Antibody Dilution: 1:100Secondary Antibody: Anti-rabbit-Alexa-546Secondary Antibody Dilution: 1:100Color/Signal Descriptions: Red: FGF2 Blue: NucleiGene Name: FGF2)

product-image-AAA199032_IHC13.jpg IHC (Immunohiostchemistry) (Sample Type: Human A375 cellsPrimary Antibody Dilution: 1:100Secondary Antibody: Anti-rabbit-Alexa-546Secondary Antibody Dilution: 1:100Color/Signal Descriptions: Red: FGF2 Blue: NucleiGene Name: FGF2)

IHC (Immunohistochemistry)

(Formalin Fixed Paraffin Embedded Tissue: Human Ovary TissueObserved Staining: NucleusPrimary Antibody Concentration: 1:600; Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3; Secondary Antibody Concentration: 1:200Magnification: 20X; Exposure Time: 0.5 - 2.0 sec)

product-image-AAA199032_IHC15.jpg IHC (Immunohistochemistry) (Formalin Fixed Paraffin Embedded Tissue: Human Ovary TissueObserved Staining: NucleusPrimary Antibody Concentration: 1:600; Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3; Secondary Antibody Concentration: 1:200Magnification: 20X; Exposure Time: 0.5 - 2.0 sec)
Related Product Information for anti-FGF2 antibody
Description of Target: The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31kDa
NCBI Official Full Name
fibroblast growth factor 2 isoform 34 kDa
NCBI Official Synonym Full Names
fibroblast growth factor 2
NCBI Official Symbol
FGF2
NCBI Official Synonym Symbols
BFGF; FGFB; FGF-2; HBGF-2
NCBI Protein Information
fibroblast growth factor 2
UniProt Protein Name
Fibroblast growth factor 2
UniProt Gene Name
FGF2
UniProt Synonym Gene Names
FGFB; FGF-2; bFGF; HBGF-2
UniProt Entry Name
FGF2_HUMAN

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Product Notes

The FGF2 fgf2 (Catalog #AAA199032) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FGF2 antibody - middle region reacts with Tested: Human, Mouse Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's FGF2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the FGF2 fgf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: RLESNNYNTY RSRKYTSWYV ALKRTGQYKL GSKTGPGQKA ILFLPMSAKS. It is sometimes possible for the material contained within the vial of "FGF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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