Rabbit FGFR2 Polyclonal Antibody | anti-FGFR2 antibody

FGFR2 Antibody (N-term)

Cat. Num. AAA28728

• Gene Names: FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
• Reactivity: Human, mouse, rat, monkey
• Applications: ELISA, Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: FGFR2, Antibody; FGFR2 Antibody (N-term); Fibroblast growth factor receptor 2; FGFR-2; K-sam; KGFR; Keratinocyte growth factor receptor; CD332; FGFR2; BEK; KSAM; anti-FGFR2 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat, monkey
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of human FGFR2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Positions: 7-37
• Sequence Length: 709

• Applicable Applications for anti-FGFR2 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: IHC~~1:50~100; IF~~1:50~100; WB~~1:1000
• Antigen Type: Synthetic Peptide
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(FGFR2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)

WB (Western Blot)

(FGFR2 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)

WB (Western Blot)

(Western blot analysis of lysates from Hela, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody R22. AAA28728 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot)

(Western blot analysis of lysates from Hela, K562, T47D cell line (from left to right), using FGFR2 Antibody (R22). AAA28728 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded H. brain section using FGFR2 Antibody (N-term). AAA28728 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

IF (Immunofluorescence)

(Fluorescent image of Hela cells stained with FGFR2 Antibody (N-term). AAA28728 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term). AAA28728 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Related Product Information for anti-FGFR2 antibody

FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in the gene are associated with many craniosynostotic syndromes and bone malformations. The genomic organization of the gene encompasses 20 exons. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.

Product Categories/Family for anti-FGFR2 antibody

Crown Antibodies; Cancer; Cardiovascular; Developmental Biology; Metabolism; Signal Transduction

References

Freeman, K.W., et al., Cancer Res. 63(19):6237-6243 (2003). Goriely, A., et al., Science 301(5633):643-646 (2003). Fomenkov, A., et al., J. Biol. Chem. 278(26):23906-23914 (2003). Katoh, M., et al., Int. J. Mol. Med. 11(5):579-583 (2003). Katoh, M., et al., Int. J. Oncol. 22(5):1155-1159 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 221316639
• NCBI GeneID: 2263
• NCBI Accession #: NP_000132.3; NP_001138385.1; NP_001138386.1; NP_001138387.1; NP_001138388.1; NP_001138389.1; NP_001138390.1; NP_001138391.1; NP_075259.4; NP_075418.1
• NCBI GenBank Nucleotide #: NM_000141.4
• UniProt Accession #: P21802; P18443; Q01742; Q12922; Q14300; Q14301; Q14302; Q14303; Q14304; B4DFC2; E7EVR6; E9PCR0
• Molecular Weight: 92025
• NCBI Official Full Name: fibroblast growth factor receptor 2 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 2
• NCBI Official Symbol: FGFR2
• NCBI Official Synonym Symbols: BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
• NCBI Protein Information: fibroblast growth factor receptor 2
• UniProt Protein Name: Fibroblast growth factor receptor 2
• UniProt Gene Name: FGFR2
• UniProt Synonym Gene Names: BEK; KGFR; KSAM; FGFR-2; KGFR
• UniProt Entry Name: FGFR2_HUMAN