Human transient receptor potential cation channel, subfamily V, member 1 ELISA Kit | TRPV1 elisa kit

Human transient receptor potential Cation channel subfamily V member 1, TrpV1 ELISA Kit

Cat. Num. AAA15589

• Reactivity: Human
• Synonyms: transient receptor potential cation channel, subfamily V, member 1; N/A; Human transient receptor potential Cation channel subfamily V member 1, TrpV1 ELISA Kit; Human transient receptor potential cation channel subfamily V member 1 (TrpV1) ELISA Kit; DKFZp434K0220; VR1; capsaicin receptor; transient receptor potential vanilloid 1a; transient receptor potential vanilloid 1b; vanilloid receptor subtype 1; transient receptor potential cation channel; subfamily V; member 1; TRPV1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human TrpV1. No significant cross-reactivity or interference between human TrpV1 and analogues was observed.
• Sequence Length: 839
• Samples: Serum, plasma, tissue homogenates, cell lysates.
• Detection Range: 0.312 ng/ml -20 ng/ml.
• Sensitivity: The minimum detectable dose of human TrpV1 is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Assay Type: Sandwich

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for TRPV1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TrpV1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TrpV1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TrpV1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TrpV1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 74315350
• NCBI GeneID: 7442
• NCBI Accession #: NP_061197.4
• NCBI GenBank Nucleotide #: NM_018727.5
• UniProt Accession #: Q8NER1; Q9H0G9; Q9H303; Q9H304; Q9NQ74; Q9NY22; A2RUA9
• Molecular Weight: 94,956 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily V member 1
• UniProt Protein Name: Transient receptor potential cation channel subfamily V member 1
• UniProt Gene Name: TRPV1
• UniProt Synonym Gene Names: VR1; TrpV1; OTRPC1
• UniProt Entry Name: TRPV1_HUMAN