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WB (Western Blot)
(Western blot analysis of FHL1 (arrow) using rabbit polyclonal FHL1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FHL1 gene.)
Rabbit anti-Human FHL1 Polyclonal Antibody | anti-FHL1 antibody
FHL1 Antibody (C-term)
Gene Names
FHL1; KYOT; SLIM; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; SLIM-1; SLIMMER
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FHL1, Antibody; FHL1 Antibody (C-term); Four and a half LIM domains protein 1; FHL-1; Skeletal muscle LIM-protein 1; SLIM; SLIM-1; FHL1; SLIM1; anti-FHL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-240 amino acids from the C-terminal region of human FHL1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
214-240
Sequence Length
296
Applicable Applications for anti-FHL1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of FHL1 (arrow) using rabbit polyclonal FHL1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FHL1 gene.)
WB (Western Blot)
(Western blot analysis of FHL1 (arrow) using rabbit polyclonal FHL1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FHL1 gene.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of widr cells using FHL1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of widr cells using FHL1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with FHL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with FHL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of FHL1 Antibody (C-term) in NIH-3T3 cell line lysates (35ug/lane). FHL1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of FHL1 Antibody (C-term) in NIH-3T3 cell line lysates (35ug/lane). FHL1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-FHL1 antibody
FHL1 is a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes.
Product Categories/Family for anti-FHL1 antibody
References
Gueneau,L., et.al., Am. J. Hum. Genet. 85 (3), 338-353 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36263
NCBI Official Full Name
four and a half LIM domains protein 1 isoform 5
NCBI Official Synonym Full Names
four and a half LIM domains 1
NCBI Official Symbol
FHL1
NCBI Official Synonym Symbols
KYOT; SLIM; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; SLIM-1; SLIMMER
NCBI Protein Information
four and a half LIM domains protein 1
UniProt Protein Name
Four and a half LIM domains protein 1
UniProt Gene Name
FHL1
UniProt Synonym Gene Names
SLIM1; FHL-1; SLIM; SLIM-1
UniProt Entry Name
FHL1_HUMAN
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Product Notes
The FHL1 fhl1 (Catalog #AAA290065) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 214-240. The FHL1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FHL1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the FHL1 fhl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FHL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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