WB (Western Blot)
(Detection of human and mouse Filamin A by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Filamin A antibody AAA211329 (lot AAA211329-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 1 second.)
Rabbit anti-Human, Mouse Filamin A Polyclonal Antibody | anti-FLNA antibody
Rabbit anti-Filamin A Antibody, Affinity Purified
Gene Names
FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Filamin A, Antibody; Rabbit anti-Filamin A Antibody, Affinity Purified; OPD1; FLN1; FLN-A; FMD; MNS; NHBP; OPD; XLVD; OPD2; XMVD; FLN; non-muscle filamin; ABP-280; filamin-A; ABPX; actin binding protein 280; Actin-binding protein 280; alpha-filamin; CSBS; endothelial actin-binding protein; epididymis secretory sperm binding protein; FGS2; filamin A, alpha; filamin-1; CVD1; Filamin-A; filamin A; anti-FLNA antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-FLNA antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1725 and 1775
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Filamin A by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Filamin A antibody AAA211329 (lot AAA211329-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 1 second.)
WB (Western Blot)
(Detection of human and mouse Filamin A by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Filamin A antibody AAA211329 (lot AAA211329-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human Filamin A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Filamin A antibody AAA211329 (lot AAA211329-1) used for IP at 3 ug/mg lysate. Filamin A was also immunoprecipitated by rabbit anti-Filamin A antibody which recognizes an upstream epitope. For blotting immunoprecipitated Filamin A, AAA211329 was used at 0.1 ug/ml. Detection: Chemiluminescence with exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human Filamin A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Filamin A antibody AAA211329 (lot AAA211329-1) used for IP at 3 ug/mg lysate. Filamin A was also immunoprecipitated by rabbit anti-Filamin A antibody which recognizes an upstream epitope. For blotting immunoprecipitated Filamin A, AAA211329 was used at 0.1 ug/ml. Detection: Chemiluminescence with exposure time of 1 second.)
IHC (Immunohistochemistry)
(Detection of human Filamin A by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Filamin A (Cat. No. AAA211329 lot 1) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Filamin A by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Filamin A (Cat. No. AAA211329 lot 1) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-FLNA antibody
Filamin A is an actin-binding protein that crosslinks and promotes orthogonal branching of actin filaments and also functions to anchor transmembrane proteins and intracellular signaling proteins to the actin cytoskeleton. As a scaffolding protein, filamin A is able to act as a link between cell membrane receptors and intracellular signaling proteins. Defects in filamin A are associated with multiple human diseases characterized by abnormalities in bone and neurological development.
Product Categories/Family for anti-FLNA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
280,018 Da
NCBI Official Full Name
filamin-A isoform 1
NCBI Official Synonym Full Names
filamin A, alpha
NCBI Official Symbol
FLNA
NCBI Official Synonym Symbols
FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
NCBI Protein Information
filamin-A; filamin-1; alpha-filamin; non-muscle filamin; actin binding protein 280; endothelial actin-binding protein
UniProt Protein Name
Filamin-A
UniProt Gene Name
FLNA
UniProt Synonym Gene Names
FLN; FLN1; FLN-A; ABP-280
UniProt Entry Name
FLNA_HUMAN
Similar Products
Product Notes
The FLNA flna (Catalog #AAA211329) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Filamin An Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Filamin A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FLNA flna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Filamin A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.