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IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I antibody at 1:200 dilution.)
Rabbit Flightless I Polyclonal Antibody | anti-fliI antibody
Flightless I antibody
Gene Names
fliI; 15; BcDNA:LD21753; CG1484; CT3691; DCA3-19; DmelCG1484; EN3; fli; fli I; fli-I; Fli-I; FLI-I; fltI; fltO; l(1)18-80; l(1)19Fd; l(1)ESHS53; l(1)W-2; lDCB2-19; sdby; w-2; W2
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Flightless I antibody was purified by antigen-affinity chromatography
Synonyms
Flightless I, Antibody; Flightless I antibody; Polyclonal Flightless I; Anti-Flightless I; Fli1; FLII; MGC39265; FLI; protein flightless-1 homolog; flightless I homolog (Drosophila); FLIL; anti-fliI antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Flightless I antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.88 mg/ml (varies by lot)
Sequence Length
1256
Applicable Applications for anti-fliI antibody
WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence)
Biological Significance
This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17.
Cross-Reactivity
Human, Mouse
Immunogen
Flightless I antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 435 and 778 of Flightless I
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I antibody at 1:200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I antibody at 1:200 dilution.)
WB (Western Blot)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 5 % SDS PAGE gel and Flightless I antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 5 % SDS PAGE gel and Flightless I antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug whole cell lysate (A:Raji) using a 5 % SDS PAGE gel and Flightless I antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug whole cell lysate (A:Raji) using a 5 % SDS PAGE gel and Flightless I antibody at a dilution of 1:1000)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless Iantibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless Iantibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Related Product Information for anti-fliI antibody
Affinity purified Rabbit polyclonal Flightless I antibody
Product Categories/Family for anti-fliI antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
145 kDa
NCBI Official Full Name
flightless I
NCBI Official Synonym Full Names
flightless I
NCBI Official Symbol
fliI
NCBI Official Synonym Symbols
15; BcDNA:LD21753; CG1484; CT3691; DCA3-19; DmelCG1484; EN3; fli; fli I; fli-I; Fli-I; FLI-I; fltI; fltO; l(1)18-80; l(1)19Fd; l(1)ESHS53; l(1)W-2; lDCB2-19; sdby; w-2; W2
NCBI Protein Information
CG1484 gene product from transcript CG1484-RA
UniProt Protein Name
Protein flightless-1
UniProt Gene Name
fliI
UniProt Entry Name
FLII_DROME
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Product Notes
The fliI flii (Catalog #AAA107807) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Flightless I can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the fliI flii for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Flightless I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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