IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FOLH1 Antibody (N-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Rabbit FOLH1 Polyclonal Antibody | anti-FOLH1 antibody
FOLH1 Antibody (N-term)
Gene Names
FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Applications
Immunohistochemistry, Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FOLH1, Antibody; FOLH1 Antibody (N-term); Glutamate carboxypeptidase 2; Cell growth-inhibiting gene 27 protein; Folate hydrolase 1; Folylpoly-gamma-glutamate carboxypeptidase; FGCP; Glutamate carboxypeptidase II; GCPII; Membrane glutamate carboxypeptidase; mGCP; N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; Prostate-specific membrane antigen; PSM; PSMA; Pteroylpoly-gamma-glutamate carboxypeptidase; FOLH1; FOLH; NAALAD1; anti-FOLH1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FOLH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from the N-terminal region of human FOLH1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
161-190
Sequence Length
442
Applicable Applications for anti-FOLH1 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FOLH1 Antibody (N-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FOLH1 Antibody (N-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(FOLH1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOLH1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(FOLH1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOLH1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(FOLH1 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the FOLH1 antibody detected the FOLH1 protein (arrow).)
WB (Western Blot)
(FOLH1 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the FOLH1 antibody detected the FOLH1 protein (arrow).)
Related Product Information for anti-FOLH1 antibody
This gene encodes a type II transmembrane glycoprotein
belonging to the M28 peptidase family. The protein acts as a
glutamate carboxypeptidase on different alternative substrates,
including the nutrient folate and the neuropeptide
N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of
tissues such as prostate, central and peripheral nervous system and
kidney. A mutation in this gene may be associated with impaired
intestinal absorption of dietary folates, resulting in low blood
folate levels and consequent hyperhomocysteinemia. Expression of
this protein in the brain may be involved in a number of
pathological conditions associated with glutamate excitotoxicity.
In the prostate the protein is up-regulated in cancerous cells and
is used as an effective prognostic indicator of
prostate cancer. This gene likely arose from a duplication event of
a nearby chromosomal region. Alternative splicing gives rise to
multiple transcript variants encoding several different isoforms.
belonging to the M28 peptidase family. The protein acts as a
glutamate carboxypeptidase on different alternative substrates,
including the nutrient folate and the neuropeptide
N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of
tissues such as prostate, central and peripheral nervous system and
kidney. A mutation in this gene may be associated with impaired
intestinal absorption of dietary folates, resulting in low blood
folate levels and consequent hyperhomocysteinemia. Expression of
this protein in the brain may be involved in a number of
pathological conditions associated with glutamate excitotoxicity.
In the prostate the protein is up-regulated in cancerous cells and
is used as an effective prognostic indicator of
prostate cancer. This gene likely arose from a duplication event of
a nearby chromosomal region. Alternative splicing gives rise to
multiple transcript variants encoding several different isoforms.
Product Categories/Family for anti-FOLH1 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Giusti, B., et al. Thromb. Haemost. 104(2):231-242(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Mlcochova, P., et al. Prostate 69(5):471-479(2009)
Davis, M.I., et al. Proc. Natl. Acad. Sci. U.S.A. 102(17):5981-5986(2005)
Giusti, B., et al. Thromb. Haemost. 104(2):231-242(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Mlcochova, P., et al. Prostate 69(5):471-479(2009)
Davis, M.I., et al. Proc. Natl. Acad. Sci. U.S.A. 102(17):5981-5986(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84331
NCBI Official Full Name
glutamate carboxypeptidase 2 isoform 2
NCBI Official Synonym Full Names
folate hydrolase (prostate-specific membrane antigen) 1
NCBI Official Symbol
FOLH1
NCBI Official Synonym Symbols
PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
NCBI Protein Information
glutamate carboxypeptidase 2
UniProt Protein Name
Glutamate carboxypeptidase 2
UniProt Gene Name
FOLH1
UniProt Synonym Gene Names
FOLH; NAALAD1; PSM; PSMA; FGCP; GCPII; mGCP; NAALADase I; PSM; PSMA
UniProt Entry Name
FOLH1_HUMAN
Similar Products
Product Notes
The FOLH1 folh1 (Catalog #AAA287536) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 161-190. The FOLH1 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's FOLH1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the FOLH1 folh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOLH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.