WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using FOXM1 antibody (AAA36882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s)
Rabbit FOXM1 Polyclonal Antibody | anti-FOXM1 antibody
FOXM1 Polyclonal Antibody
Gene Names
FOXM1; MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2
Applications
Western Blot
Purity
Affinity Purification
Synonyms
FOXM1, Antibody; FOXM1 Polyclonal Antibody; FKHL16; FOXM1B; HFH-11; HFH11; HNF-3; INS-1; MPHOSPH2; MPP-2; MPP2; PIG29; TGT3; TRIDENT; anti-FOXM1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Sequence Length
763
Applicable Applications for anti-FOXM1 antibody
WB (Western Blot)
Category
Primary Antibody
Species Reactivity/Cross Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human FOXM1 (NP_973732.1)
Preparation and Storage
Store at -20°C . Avoid freeze / thaw cycles.
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using FOXM1 antibody (AAA36882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using FOXM1 antibody (AAA36882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s)
Related Product Information for anti-FOXM1 antibody
Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis and cell proliferation. FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3). Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4). Phosphorylation of FoxM1 by MAPK (Ser331 Ser704), Cyclin/Cdk (Thr600, Thr611, Thr672), Plk1 (Ser715, Ser724), and Chk2 (Ser376) stabilizes and activates FoxM1 (4-7). Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (8). Recent studies have shown that FoxM1 expression is negatively regulated by p53 (9,10). Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver, and kidney, and may be associated with p53 mutations in some tumors (10,11). As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (12).
Product Categories/Family for anti-FOXM1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 110 kDa
Calculated MW: 82 kDa/84 kDa/ 88 kDa
Calculated MW: 82 kDa/84 kDa/ 88 kDa
NCBI Official Full Name
Forkhead box protein M1
NCBI Official Synonym Full Names
forkhead box M1
NCBI Official Symbol
FOXM1
NCBI Official Synonym Symbols
MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2
NCBI Protein Information
forkhead box protein M1; M-phase phosphoprotein 2; HNF-3/fork-head homolog 11; transcription factor Trident; MPM-2 reactive phosphoprotein 2; forkhead-related protein FKHL16; winged-helix factor from INS-1 cells; Forkhead, drosophila, homolog-like 16; hepatocyte nuclear factor 3 forkhead homolog 11
UniProt Protein Name
Forkhead box protein M1
UniProt Gene Name
FOXM1
UniProt Synonym Gene Names
FKHL16; HFH11; MPP2; WIN; HFH-11; HNF-3/fork-head homolog 11
UniProt Entry Name
FOXM1_HUMAN
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Product Notes
The FOXM1 foxm1 (Catalog #AAA36882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FOXM1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the FOXM1 foxm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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