WB (Western Blot)
(FOXN3 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FOXN3 antibody detected the FOXN3 protein (arrow).)
Rabbit anti-Human FOXN3 Polyclonal Antibody | anti-FOXN3 antibody
FOXN3 Antibody (C-term)
Gene Names
FOXN3; CHES1; PRO1635; C14orf116
Reactivity
Human
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FOXN3, Antibody; FOXN3 Antibody (C-term); Forkhead box protein N3; Checkpoint suppressor 1; FOXN3; C14orf116; CHES1; anti-FOXN3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FOXN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-408 amino acids from the C-terminal region of human FOXN3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
380-408
Sequence Length
468
Applicable Applications for anti-FOXN3 antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(FOXN3 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FOXN3 antibody detected the FOXN3 protein (arrow).)
WB (Western Blot)
(FOXN3 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FOXN3 antibody detected the FOXN3 protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of Hela cell stained with FOXN3 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).FOXN3 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent image of Hela cell stained with FOXN3 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).FOXN3 immunoreactivity is localized to Nucleus significantly.)
Related Product Information for anti-FOXN3 antibody
This gene is a member of the forkhead/winged helix
transcription factor family. Checkpoints are eukaryotic DNA
damage-inducible cell cycle arrests at G1 and G2. Checkpoint
suppressor 1 suppresses multiple yeast checkpoint mutations
including mec1, rad9, rad53 and dun1 by activating a
MEC1-independent checkpoint pathway. Alternative splicing is
observed at the locus, resulting in distinct isoforms. [provided by
RefSeq].
transcription factor family. Checkpoints are eukaryotic DNA
damage-inducible cell cycle arrests at G1 and G2. Checkpoint
suppressor 1 suppresses multiple yeast checkpoint mutations
including mec1, rad9, rad53 and dun1 by activating a
MEC1-independent checkpoint pathway. Alternative splicing is
observed at the locus, resulting in distinct isoforms. [provided by
RefSeq].
References
Samaan, G., et al. Biochem. Biophys. Res. Commun. 400(1):60-65(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Markowski, J., et al. Eur Arch Otorhinolaryngol 266(10):1501-1507(2009)
Schlade-Bartusiak, K., et al. Am. J. Med. Genet. A 146A (1), 117-123 (2008) :
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Markowski, J., et al. Eur Arch Otorhinolaryngol 266(10):1501-1507(2009)
Schlade-Bartusiak, K., et al. Am. J. Med. Genet. A 146A (1), 117-123 (2008) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53835
NCBI Official Full Name
forkhead box protein N3 isoform 1
NCBI Official Synonym Full Names
forkhead box N3
NCBI Official Symbol
FOXN3
NCBI Official Synonym Symbols
CHES1; PRO1635; C14orf116
NCBI Protein Information
forkhead box protein N3
UniProt Protein Name
Forkhead box protein N3
UniProt Gene Name
FOXN3
UniProt Synonym Gene Names
C14orf116; CHES1
UniProt Entry Name
FOXN3_HUMAN
Similar Products
Product Notes
The FOXN3 foxn3 (Catalog #AAA286183) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 380-408. The FOXN3 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOXN3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the FOXN3 foxn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXN3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.