IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FOXO3 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Rabbit FOXO3 Polyclonal Antibody | anti-FOXO3 antibody
FOXO3 Antibody (Center)
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FOXO3, Antibody; FOXO3 Antibody (Center); Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1; FOXO3A; anti-FOXO3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FOXO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-253 amino acids from the Central region of human FOXO3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
224-253
Sequence Length
453
Applicable Applications for anti-FOXO3 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FOXO3 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FOXO3 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(FOXO3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B FOXO3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(FOXO3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B FOXO3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(FOXO3 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FOXO3 antibody detected the FOXO3 protein (arrow).)
WB (Western Blot)
(FOXO3 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FOXO3 antibody detected the FOXO3 protein (arrow).)
Related Product Information for anti-FOXO3 antibody
This gene belongs to the forkhead family of transcription
factors which are characterized by a distinct forkhead domain. This
gene likely functions as a trigger for apoptosis through expression
of genes necessary for cell death. Translocation of this gene with
the MLL gene is associated with secondary acute leukemia.
Alternatively spliced transcript variants encoding the same protein
have been observed.
factors which are characterized by a distinct forkhead domain. This
gene likely functions as a trigger for apoptosis through expression
of genes necessary for cell death. Translocation of this gene with
the MLL gene is associated with secondary acute leukemia.
Alternatively spliced transcript variants encoding the same protein
have been observed.
Product Categories/Family for anti-FOXO3 antibody
References
Zhuo de, X., et al. J. Biol. Chem. 285(41):31491-31501(2010)
Tudzarova, S., et al. EMBO J. 29(19):3381-3394(2010)
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Mikse, O.R., et al. Cancer Res. 70(15):6205-6215(2010)
Chen, J., et al. PLoS ONE 5 (8), E12293 (2010) :
Tudzarova, S., et al. EMBO J. 29(19):3381-3394(2010)
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Mikse, O.R., et al. Cancer Res. 70(15):6205-6215(2010)
Chen, J., et al. PLoS ONE 5 (8), E12293 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71277
NCBI Official Full Name
forkhead box protein O3
NCBI Official Synonym Full Names
forkhead box O3
NCBI Official Symbol
FOXO3
NCBI Official Synonym Symbols
FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
NCBI Protein Information
forkhead box protein O3
UniProt Protein Name
Forkhead box protein O3
UniProt Gene Name
FOXO3
UniProt Synonym Gene Names
FKHRL1; FOXO3A
UniProt Entry Name
FOXO3_HUMAN
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Product Notes
The FOXO3 foxo3 (Catalog #AAA284995) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 224-253. The FOXO3 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's FOXO3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the FOXO3 foxo3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXO3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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