WB (Western Blot)
(Figure 2. Western blot analysis of FSTL3 using anti- FSTL3antibody (AAA45376).Lane 1: rat liver tissue lysatesLane 2: rat testis tissue lysatesLane 3: rat NRK whole cell lysatesLane 4: mouse liver tissue lysatesLane 5: mouse testis tissue lysatesLane 6: mouse lung tissue lysates.)
Rabbit FSTL3 Polyclonal Antibody | anti-FSTL3 antibody
Anti-FSTL3 antibody
Gene Names
FSTL3; FLRG; FSRP
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
FSTL3, Antibody; Anti-FSTL3 antibody; Follistatin-related protein 3; follistatin-like 3(secreted glycoprotein); FLRG antibody; follistatin-like 3 antibody; follistatin-like 3 glycoprotein antibody; Follistatin-like protein 3 antibody; Follistatin-related gene protein antibody; Follistatin-related protein 3 antibody; FSRP antibody; Fstl3 antibody; FSTL3_HUMAN antibody; anti-FSTL3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
263
Applicable Applications for anti-FSTL3 antibody
WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human FSTL3 (145-159aa ECELRAARCRGHPDL), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 2. Western blot analysis of FSTL3 using anti- FSTL3antibody (AAA45376).Lane 1: rat liver tissue lysatesLane 2: rat testis tissue lysatesLane 3: rat NRK whole cell lysatesLane 4: mouse liver tissue lysatesLane 5: mouse testis tissue lysatesLane 6: mouse lung tissue lysates.)
WB (Western Blot)
(Figure 2. Western blot analysis of FSTL3 using anti- FSTL3antibody (AAA45376).Lane 1: rat liver tissue lysatesLane 2: rat testis tissue lysatesLane 3: rat NRK whole cell lysatesLane 4: mouse liver tissue lysatesLane 5: mouse testis tissue lysatesLane 6: mouse lung tissue lysates.)
WB (Western Blot)
(Figure 1. Western blot analysis of FSTL3 using anti- FSTL3antibody (AAA45376).Lane 1: human U2OS whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human PC-3 whole cell lysatesLane 5: human HepG2 whole cell lysatesLane 6: human K562 whole cell lysates.)
WB (Western Blot)
(Figure 1. Western blot analysis of FSTL3 using anti- FSTL3antibody (AAA45376).Lane 1: human U2OS whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human PC-3 whole cell lysatesLane 5: human HepG2 whole cell lysatesLane 6: human K562 whole cell lysates.)
Related Product Information for anti-FSTL3 antibody
Description: Rabbit IgG polyclonal antibody for Follistatin-related protein 3(FSTL3) detection. Tested with WB in Human, Mouse, Rat.
Background: FSTL3(Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins though to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. By Southern blot analysis of somatic cell hybrids and FISH, Hayette et al.(1998) localized the FSTL3 gene to chromosome 19p13. Using recombinant mouse Fstl3, Tsuchida et al.(2000) found that Fstl3bound both activin and BMP2 and had a higher affinity for activin. Overexpression of Fstl3 inhibited BMP2-induced cell signaling in a reporter assay. In addition to the translocation in a case of B-cell chronic lymphocytic leukemia from which FSTL3 was isolated, Hayette et al.(1998) also observed rearrangement of the FSTL3 gene in a case of B-cell non-Hodgkin lymphoma and in a case of B-cell mantle zone lymphoma, suggesting that FSTL3 may be involved in the leukemogenesis process.
Background: FSTL3(Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins though to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. By Southern blot analysis of somatic cell hybrids and FISH, Hayette et al.(1998) localized the FSTL3 gene to chromosome 19p13. Using recombinant mouse Fstl3, Tsuchida et al.(2000) found that Fstl3bound both activin and BMP2 and had a higher affinity for activin. Overexpression of Fstl3 inhibited BMP2-induced cell signaling in a reporter assay. In addition to the translocation in a case of B-cell chronic lymphocytic leukemia from which FSTL3 was isolated, Hayette et al.(1998) also observed rearrangement of the FSTL3 gene in a case of B-cell non-Hodgkin lymphoma and in a case of B-cell mantle zone lymphoma, suggesting that FSTL3 may be involved in the leukemogenesis process.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
24,960 Da
NCBI Official Full Name
Follistatin-related protein 3
NCBI Official Synonym Full Names
follistatin-like 3 (secreted glycoprotein)
NCBI Official Symbol
FSTL3
NCBI Official Synonym Symbols
FLRG; FSRP
NCBI Protein Information
follistatin-related protein 3; follistatin-like protein 3; follistatin-related gene protein
UniProt Protein Name
Follistatin-related protein 3
UniProt Gene Name
FSTL3
UniProt Synonym Gene Names
FLRG
UniProt Entry Name
FSTL3_HUMAN
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Product Notes
The FSTL3 fstl3 (Catalog #AAA45376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FSTL3 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FSTL3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the FSTL3 fstl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FSTL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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