WB (Western Blot)
(Detection of human and mouse FUS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-FUS antibody AAA212307 (AAA212307 Lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Goat anti-Human, Mouse FUS Polyclonal Antibody | anti-FUS antibody
Goat anti-FUS Antibody, Affinity Purified
Gene Names
FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
FUS, Antibody; Goat anti-FUS Antibody, Affinity Purified; TLS; HNRNPP2; oncogene FUS; translocated in liposarcoma protein; oncogene TLS; FUS/ERG chimeric protein; RNA-binding protein FUS; heterogeneous nuclear ribonucleoprotein P2; POMP75; fusion gene in myxoid liposarcoma; fused in sarcoma; FUS/ERG fusion protein; ETM4; ALS6; 75 kDa DNA-pairing protein; fus-like protein; FUS1; FUS RNA binding protein; anti-FUS antibody
Host
Goat
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-FUS antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse FUS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-FUS antibody AAA212307 (AAA212307 Lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse FUS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-FUS antibody AAA212307 (AAA212307 Lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human FUS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-FUS antibody AAA212307 (AAA212307 Lot 2) used for IP at 6 ug per reaction. FUS was also immunoprecipitated by a previous lot of this antibody (lot AAA212307-1) and rabbit anti-FUS antibody For blotting immunoprecipitated FUS, AAA212307-2 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human FUS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-FUS antibody AAA212307 (AAA212307 Lot 2) used for IP at 6 ug per reaction. FUS was also immunoprecipitated by a previous lot of this antibody (lot AAA212307-1) and rabbit anti-FUS antibody For blotting immunoprecipitated FUS, AAA212307-2 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IHC (Immunohistochemistry)
(Detection of human FUS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified goat anti-FUS (Cat. No. AAA212307 lot 2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human FUS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified goat anti-FUS (Cat. No. AAA212307 lot 2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-FUS antibody
FUS has been identified as a frequent translocation fusion partner of various transcription factors. FUS fusion genes have been shown to be associated with multiple tumor types which include liposarcoma, leukemia, histocytoma, and sarcoma. FUS is a multifunctional RNA-binding protein that associates with the nuclear matrix and Cajal bodies and appears to play a role in splicesome assembly, pre-mRNA splicing, DNA repair, transcriptional regulation and homologous recombination.
Product Categories/Family for anti-FUS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,355 Da
NCBI Official Full Name
RNA-binding protein FUS isoform 1
NCBI Official Synonym Full Names
FUS RNA binding protein
NCBI Official Symbol
FUS
NCBI Official Synonym Symbols
TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
NCBI Protein Information
RNA-binding protein FUS
UniProt Protein Name
RNA-binding protein FUS
UniProt Gene Name
FUS
UniProt Synonym Gene Names
TLS
UniProt Entry Name
FUS_HUMAN
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Product Notes
The FUS fus (Catalog #AAA212307) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FUS Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FUS can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FUS fus for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FUS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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