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IF (Immunofluorescence)
(Figure 4 Immunofluorescence Validation of FZD6 in Rat LungImmunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with AAA40965 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Rabbit FZD6 Polyclonal Antibody | anti-FZD6 antibody
FZD6 (IN) Antibody
Gene Names
FZD6; FZ6; FZ-6; HFZ6; NDNC10
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot, ELISA
Purity
FZD6 Antibody is affinity chromatography purified via peptide column.
Synonyms
FZD6, Antibody; FZD6 (IN) Antibody; FZD6 Antibody: Frizzled-6; Fz-6; hFz6; frizzled class receptor 6; anti-FZD6 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
FZD6 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; FZD6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-FZD6 antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen
Anti-FZD6 antibody (9551) was raised against a peptide corresponding to 14 amino acids in the central domain of human FZD6.
The immunogen is located within 50-550 amino acids of FZD6.
The immunogen is located within 50-550 amino acids of FZD6.
Homology
Predicted species reactivity based on immunogen sequence: Chimpanzee(100%); Monkey (100%); Dog (100%)
Research Area
Cell Cycle, Signal Transduction, Stem Cell, Growth Factors
NCBI Organism
Homo sapiens
Isoforms
Human FZD6 has 2 isoforms, including isoform 1 (706aa, 79.3kD) and isoform 2 (674aa, 75.6kD). Mouse FZD6 has 1 isoform (709aa, 79kD). Rat FZD6 has one isoform (710aa, 79.1kD). can detect FZD6 in human, mouse and rat.
Preparation and Storage
FZD6 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Figure 4 Immunofluorescence Validation of FZD6 in Rat LungImmunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with AAA40965 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 4 Immunofluorescence Validation of FZD6 in Rat LungImmunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with AAA40965 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 3 Immunofluorescence Validation of FZD6 in Mouse LungImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling FZD6 with AAA40965 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 3 Immunofluorescence Validation of FZD6 in Mouse LungImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling FZD6 with AAA40965 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 2 Immunofluorescence Validation of FZD6 in Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling FZD6 with AAA40965 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 2 Immunofluorescence Validation of FZD6 in Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling FZD6 with AAA40965 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
WB (Western Blot)
(Figure 1 WB Validation in Human, Mouse and Rat Cell LinesLoading: 15ug of lysate. Antibodies: FZD6 AAA40965, 2ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1 WB Validation in Human, Mouse and Rat Cell LinesLoading: 15ug of lysate. Antibodies: FZD6 AAA40965, 2ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.)
Related Product Information for anti-FZD6 antibody
FZD6 Antibody: FZD6 acts as a receptor for Wnt proteins. Frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Together with FZD3, FZD6 is involved with neural tube closure and plays a role in the regulation of planar cell polarity, particularly in the orientation of asymmetric bundles of stereocilia on apical faces of a subset of sensory cells located in the inner ear.
Product Categories/Family for anti-FZD6 antibody
References
Wang et al. J. Neurosci. 206;26(8):2147-2156
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,292 Da
NCBI Official Full Name
frizzled-6 isoform a
NCBI Official Synonym Full Names
frizzled family receptor 6
NCBI Official Symbol
FZD6
NCBI Official Synonym Symbols
FZ6; FZ-6; HFZ6; NDNC10
NCBI Protein Information
frizzled-6; frizzled homolog 6; seven transmembrane helix receptor; frizzled 6, seven transmembrane spanning receptor
UniProt Protein Name
Frizzled-6
UniProt Gene Name
FZD6
UniProt Synonym Gene Names
Fz-6; hFz6
UniProt Entry Name
FZD6_HUMAN
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Product Notes
The FZD6 fzd6 (Catalog #AAA40965) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FZD6 (IN) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FZD6 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the FZD6 fzd6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FZD6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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