Rabbit anti-Human, Mouse FZD9 Polyclonal Antibody | anti-FZD9 antibody

FZD9 Antibody

Cat. Num. AAA232493

• Gene Names: FZD9; FZD3; CD349
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: FZD9, Antibody; FZD9 Antibody; Frizzled-9; Fz-9; hFz9; FzE6; CD349; FZD9; FZD3, (CD Antigen CD349); anti-FZD9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-FZD9 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant Human Frizzled-9 protein (23-229AA)
• Conjugate: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Species: Homo sapiens (Human)
• Research Area: Neuroscience
• Target Names: FZD9
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

IHC (Immunohiostchemistry)

(IHC image of AAA232493 diluted at 1:500 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot)

(Western BlotPositive WB detected in:Mouse liver tissue,Mouse kidney tissueAll lanes: FZD9 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 65 kDaObserved band size: 65 kDa)

Related Product Information for anti-FZD9 antibody

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the betacatenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 4503835
• NCBI GeneID: 8326
• NCBI Accession #: NP_003499.1
• NCBI GenBank Nucleotide #: NM_003508.2
• UniProt Accession #: O00144
• NCBI Official Full Name: frizzled-9
• NCBI Official Synonym Full Names: frizzled class receptor 9
• NCBI Official Symbol: FZD9
• NCBI Official Synonym Symbols: FZD3; CD349
• NCBI Protein Information: frizzled-9
• UniProt Protein Name: Frizzled-9
• UniProt Gene Name: FZD9
• UniProt Synonym Gene Names: FZD3; Fz-9; hFz9
• UniProt Entry Name: FZD9_HUMAN