Rabbit Gab2 Polyclonal Antibody | anti-Gab2 antibody

Gab2 Antibody

Cat. Num. AAA329570

• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (92%), Horse (100%), Sheep (92%), Rabbit (100%), Dog (100%), Xenopus (100%)
• Applications: ELISA, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: Gab2, Antibody; Gab2 Antibody; GAB 2; Gab2; GAB2_HUMAN; Grb 2 associated binder 2; GRB 2 associated binding protein 2; Grb2 associated binder 2; GRB2 associated binder 2 pp100; GRB2 associated binding protein 2; GRB2-associated binder 2; GRB2-associated-binding protein 2; Growth factor receptor bound protein 2 associated protein 2; Growth factor receptor bound protein 2-associated protein 2; KIAA0571; p97; pHdomain containing adaptor molecule p97; pp100; anti-Gab2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (92%), Horse (100%), Sheep (92%), Rabbit (100%), Dog (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Gab2 Antibody detects endogenous levels of total Gab2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Gab2 antibody: ELISA (Peptide ELISA), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human Gab2, corresponding to a region within the internal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated on tyrosine residue (s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.Dephosphorylated by PTPN11.
• Subunit Structure: Interacts with SHC1; may mediate interaction with receptors (By similarity). Interacts with SYK (By similarity). Interacts with PI-3 kinase. Interacts with GRB2 (via SH3 2 domain). Interacts (phosphorylated) with PTPN11. Interacts with TNFRSF11A (via cytoplasmic domain). Interacts (phosphorylated) with 14-3-3 family proteins SFN, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ and YWHAZ; prevents interaction with GRB2 and attenuates GAB2 signaling. Interacts with HCK.
• Similarity: The SH3-binding motifs mediate interaction with SHC1 and GRB2.The pHdomain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.Belongs to the GAB family.
• Subcellular Location: Cytoplasm. Cell membrane.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of Gab2 in lysates of MCF7, using Gab2 Antibody(AF4803).)

WB (Western Blot)

(Western blot analysis of extracts from B16F10 cells, using Gab2 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-Gab2 antibody

Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.

NCBI and Uniprot Product Information

• NCBI GI #: 6912460
• NCBI GeneID: 9846
• NCBI Accession #: NP_036428.1
• NCBI GenBank Nucleotide #: NM_012296.3
• UniProt Accession #: Q9UQC2; O60317; A2RRM2; A6NEW9; A7MD36
• Molecular Weight: 74,458 Da
• NCBI Official Full Name: GRB2-associated-binding protein 2 isoform b
• NCBI Official Synonym Full Names: GRB2-associated binding protein 2
• NCBI Official Symbol: GAB2
• NCBI Protein Information: GRB2-associated-binding protein 2; pp100; Grb2-associated binder 2; growth factor receptor bound protein 2-associated protein 2
• UniProt Protein Name: GRB2-associated-binding protein 2
• UniProt Gene Name: GAB2
• UniProt Synonym Gene Names: KIAA0571
• UniProt Entry Name: GAB2_HUMAN