IHC (Immunohiostchemistry)
(Staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit GABBR2 Polyclonal Antibody | anti-GABBR2 antibody
Phospho-GABBR2 (Ser884) Antibody
Gene Names
GABBR2; HG20; GPR51; GPRC3B; GABABR2; HRIHFB2099
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
GABBR2, Antibody; Phospho-GABBR2 (Ser884) Antibody; BcDNA:GH07312; CG6706; CT20836; D Gaba2; FLJ36928; G protein coupled receptor 51; G-protein coupled receptor 51; GAB B R2; GABA B R2; GABA B receptor 2; GABA-B receptor 2; GABA-B-R2; GABA-BR2; GABAB R2; GABABR 2; GABABR2; GABB R2; GABBR 2; Gabbr2; GABR2_HUMAN; Gamma aminobutyric acid B receptor 2; Gamma aminobutyric acid GABA B receptor 2; Gamma aminobutyric acid type B receptor subunit 2; Gamma-aminobutyric acid type B receptor subunit 2; Gb 2; Gb2; GH07312; GPR 51; GPR51; GPRC 3B; GPRC3B; HG 20; HG20; HRIHFB2099; Metabotropic GABA B receptor subtype 2; OTTHUMP00000021776; OTTHUMP00000063797; R2 SUBUNIT; anti-GABBR2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-GABBR2 (Ser884) Antibody detects endogenous levels of GABBR2 only when phosphorylated at Ser884.
Tissue Specificity: Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.
Tissue Specificity: Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GABBR2 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human GABBR2 around the phosphorylation site of Ser884.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Heterodimer of GABBR1 and GABBR2. Homodimers may form, but are inactive. Interacts (via C-terminus) with ATF4 (via leucine zipper domain) (By similarity).
Similarity
Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABBR1.Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
Subcellular Location
Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein.
Note: Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. In contrast, GABBR2 does not depend on GABBR1 for transport to the cell membrane.
Note: Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. In contrast, GABBR2 does not depend on GABBR1 for transport to the cell membrane.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(Staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis GABBR2 (Phospho-Ser884) using UV treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis GABBR2 (Phospho-Ser884) using UV treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-GABBR2 antibody
Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
105,821 Da
NCBI Official Full Name
gamma-aminobutyric acid type B receptor subunit 2
NCBI Official Synonym Full Names
gamma-aminobutyric acid (GABA) B receptor, 2
NCBI Official Symbol
GABBR2
NCBI Official Synonym Symbols
HG20; GPR51; GPRC3B; GABABR2; HRIHFB2099
NCBI Protein Information
gamma-aminobutyric acid type B receptor subunit 2; gb2; GABA-BR2; GABA-B-R2; GABA-B receptor 2; GABA-B receptor, R2 subunit; G protein-coupled receptor 51; G-protein coupled receptor 51; gamma-aminobutyric acid B receptor 2
UniProt Protein Name
Gamma-aminobutyric acid type B receptor subunit 2
UniProt Gene Name
GABBR2
UniProt Synonym Gene Names
GPR51; GPRC3B; GABA-B receptor 2; GABA-B-R2; GABA-BR2; GABABR2; Gb2
UniProt Entry Name
GABR2_HUMAN
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Product Notes
The GABBR2 gabbr2 (Catalog #AAA329870) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-GABBR2 (Ser884) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's GABBR2 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GABBR2 gabbr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GABBR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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