FCM/FACS (Flow Cytometry)
(GALNT4 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit GALNT4 Polyclonal Antibody | anti-GALNT4 antibody
GALNT4 Antibody (N-term)
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GALNT4, Antibody; GALNT4 Antibody (N-term); Polypeptide N-acetylgalactosaminyltransferase 4; Polypeptide GalNAc transferase 4; GalNAc-T4; pp-GaNTase 4; Protein-UDP acetylgalactosaminyltransferase 4; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4; GALNT4; anti-GALNT4 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GALNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of human GALNT4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
93-120
Sequence Length
406
Applicable Applications for anti-GALNT4 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GALNT4 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GALNT4 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(GALNT4 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GALNT4 antibody detected the GALNT4 protein (arrow).)
WB (Western Blot)
(GALNT4 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GALNT4 antibody detected the GALNT4 protein (arrow).)
Related Product Information for anti-GALNT4 antibody
This gene encodes a member of the
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes.
GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi
apparatus by catalyzing the transfer of GalNAc to serine and
threonine residues on target proteins. They are characterized by an
N-terminal transmembrane domain, a stem region, a lumenal catalytic
domain containing a GT1 motif and Gal/GalNAc transferase motif, and
a C-terminal ricin/lectin-like domain. GalNAc-Ts have different,
but overlapping, substrate specificities and patterns of
expression. In vitro, the encoded protein can complement other
GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem
repeat and can O-glycosylate the P-selectin glycoprotein ligand-1
molecule. The coding region of this gene is contained within a
single exon.
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes.
GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi
apparatus by catalyzing the transfer of GalNAc to serine and
threonine residues on target proteins. They are characterized by an
N-terminal transmembrane domain, a stem region, a lumenal catalytic
domain containing a GT1 motif and Gal/GalNAc transferase motif, and
a C-terminal ricin/lectin-like domain. GalNAc-Ts have different,
but overlapping, substrate specificities and patterns of
expression. In vitro, the encoded protein can complement other
GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem
repeat and can O-glycosylate the P-selectin glycoprotein ligand-1
molecule. The coding region of this gene is contained within a
single exon.
References
O'Halloran, A.M., et al. J. Thromb. Thrombolysis 27(2):175-184(2009)
Argueso, P., et al. Invest. Ophthalmol. Vis. Sci. 44(1):86-92(2003)
Hassan, H., et al. J. Biol. Chem. 275(49):38197-38205(2000)
Bennett, E.P., et al. J. Biol. Chem. 273(46):30472-30481(1998)
Bennett, E.P., et al. Glycobiology 8(6):547-555(1998)
Argueso, P., et al. Invest. Ophthalmol. Vis. Sci. 44(1):86-92(2003)
Hassan, H., et al. J. Biol. Chem. 275(49):38197-38205(2000)
Bennett, E.P., et al. J. Biol. Chem. 273(46):30472-30481(1998)
Bennett, E.P., et al. Glycobiology 8(6):547-555(1998)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66666
NCBI Official Full Name
POC1B-GALNT4 protein isoform 2
NCBI Official Synonym Full Names
POC1B-GALNT4 readthrough
NCBI Official Symbol
POC1B-GALNT4
NCBI Protein Information
POC1B-GALNT4 protein
UniProt Protein Name
Polypeptide N-acetylgalactosaminyltransferase 4
UniProt Gene Name
GALNT4
UniProt Synonym Gene Names
GalNAc-T4; pp-GaNTase 4
UniProt Entry Name
GALT4_HUMAN
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Product Notes
The GALNT4 galnt4 (Catalog #AAA290059) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 93-120. The GALNT4 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's GALNT4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GALNT4 galnt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GALNT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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