WB (Western Blot)
((0. 03ug/ml) staining of lysates of cell line HEK293 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat GAPDH Polyclonal Antibody | anti-GAPDH antibody
Goat anti-GAPDH (C Terminus) Loading Control Antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Dog, Pig
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
GAPDH, Antibody; Goat anti-GAPDH (C Terminus) Loading Control Antibody; Goat Anti-GAPDH (Internal), Biotinylated Antibody; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; HGNC: 4141; G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432; aging-associated gene 9 protein; glyceraldehyde 3-phosphate dehydrogenase; GAPDH antibody; glyceraldehyde-3-phosphate dehydrogenase antibody; HGNC: 4141 antibody; G3PD antibody; GAPD antibody; MGC88685 antibody; OTTHUMP00000174431 antibody; OTTHUMP00000174432 antibody; aging-associated gene 9 protein antibody; glyceraldehyde 3-phosphate dehydrogenase antibody; GAPDH (C Terminus) Loading Control; anti-GAPDH antibody
Host
Goat
Reactivity
Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Dog, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
HQVVSSDFNSDT
Sequence Length
335
Applicable Applications for anti-GAPDH antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
Epitope
C Terminus
Note
GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-20357
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
WB (Western Blot)
((0. 03ug/ml) staining of lysates of cell line HEK293 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((0. 03ug/ml) staining of lysates of cell line HEK293 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((0.1ug/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated GAPDH knock-down expresson in right lane) (35ug protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((0.1ug/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated GAPDH knock-down expresson in right lane) (35ug protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.)
References
Henson MA, Roberts AC, Salimi K, Vadlamudi S, Hamer RM, Gilmore JH, Jarskog LF, Philpot BD.; Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex.; Cereb Cortex.2008 Nov;18(11): 2560-73.; Pubmed 18296432
This antibody has been successfully used in the following paper: ; Kiepe D, Van Der Pas A, Ciarmatori S, St¤ndker L, Schtt B, Hoeflich A, Hgel U, Oh J, T¶nshoff B.; Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein 2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I.; Endocrinology.2008 Oct;149(10): 4901-11.; Pubmed 18556354
The goat polyclonal antibody used as described in this paper was manufactured by us: ; Gerstner JR, Bremer QZ, Vander Heyden WM, Lavaute TM, Yin JC, Landry CF.; Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain.; PLoS One.2008 Feb 20;3(2): e1631.; Pubmed 18286188
* The goat polyclonal antibody used as described in this paper was manufactured by us: ; Nguyen M, Pace AJ, Koller BH.; Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.; J Exp Med.2007 Jun 11;204(6): 1383-93.; Pubmed 17517966
This antibody has been successfully used in the following paper: ; Kiepe D, Van Der Pas A, Ciarmatori S, St¤ndker L, Schtt B, Hoeflich A, Hgel U, Oh J, T¶nshoff B.; Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein 2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I.; Endocrinology.2008 Oct;149(10): 4901-11.; Pubmed 18556354
The goat polyclonal antibody used as described in this paper was manufactured by us: ; Gerstner JR, Bremer QZ, Vander Heyden WM, Lavaute TM, Yin JC, Landry CF.; Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain.; PLoS One.2008 Feb 20;3(2): e1631.; Pubmed 18286188
* The goat polyclonal antibody used as described in this paper was manufactured by us: ; Nguyen M, Pace AJ, Koller BH.; Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.; J Exp Med.2007 Jun 11;204(6): 1383-93.; Pubmed 17517966
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,548 Da
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 1
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD; HEL-S-162eP
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
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Product Notes
The GAPDH gapdh (Catalog #AAA60874) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GAPDH (C Terminus) Loading Control Antibody reacts with Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HQVVSSDFNS DT. It is sometimes possible for the material contained within the vial of "GAPDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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