WB (Western Blot)
(Western blot analysis of different cell cytosolic or nuclear enriched fractions, using rabbit pAb to GAPDH, AAA76738, dilution 1:20,000, in red: [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions.)
Rabbit GAPDH Polyclonal Antibody | anti-GAPDH antibody
GAPDH
Gene Names
GAPDH; GAPD
Reactivity
Human, Mouse, Rat, Chicken, Bovine, Porcine, Equine
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
Synonyms
GAPDH, Antibody; GAPDH; anti-GAPDH antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Bovine, Porcine, Equine
Clonality
Polyclonal
Isotype
Rabbit IgG
Form/Format
Liquid. Antibody is supplied as analiquot of serum plus 5mMNaN3
Applicable Applications for anti-GAPDH antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Full length recombinant human GAPDH expressed in and purified from E coli
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of different cell cytosolic or nuclear enriched fractions, using rabbit pAb to GAPDH, AAA76738, dilution 1:20,000, in red: [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions.)
WB (Western Blot)
(Western blot analysis of different cell cytosolic or nuclear enriched fractions, using rabbit pAb to GAPDH, AAA76738, dilution 1:20,000, in red: [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells stained with rabbit pAb to GAPDH, AAA76738, dilution 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. AAA76738 produces diffuse cytoplasmic staining of cells.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells stained with rabbit pAb to GAPDH, AAA76738, dilution 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. AAA76738 produces diffuse cytoplasmic staining of cells.)
Related Product Information for anti-GAPDH antibody
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate to produce glyceraldehyde 1-3-bisphosphate. Because GAPDH protein is expressed in large amounts and is required at all times for an important "house keeping" function, levels of GAPDH mRNA are often measured and used as standards in studies of the expression of other mRNAs. Similarly specific antibodies to GAPDH are used to measure GAPDH expression as a protein standard in western blotting experiments. This antibody can be used as a loading control for such experiments, allowing comparison between the expression level of this protein and others in a cell or tissue.
The immunogen used to raise RA22148 was extensively purified pig GAPDH. The antibody has been widely used as a western blotting standard by many labs for many years and is known to detect GAPDH in a variety of mammalian species including human, rat and mouse. It produces a single clean band on western blots of typical cell and tissue extracts.
The immunogen used to raise RA22148 was extensively purified pig GAPDH. The antibody has been widely used as a western blotting standard by many labs for many years and is known to detect GAPDH in a variety of mammalian species including human, rat and mouse. It produces a single clean band on western blots of typical cell and tissue extracts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,836 Da
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
GAPD
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
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Product Notes
The GAPDH gapdh (Catalog #AAA76738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAPDH reacts with Human, Mouse, Rat, Chicken, Bovine, Porcine, Equine and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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