IP (Immunoprecipitation)
(GAPDH was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate Lane B:0.5 mg H1299 Whole Cell Lysate 4 uL anti- GAPDH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- GAPDH rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36kDa Observed band size :36 kDa)
Rabbit anti-Human GAPDH Polyclonal Antibody | anti-GAPDH antibody
Anti-GAPDH Antibody, Rabbit Polyclonal
Gene Names
GAPDH; G3PD; GAPD
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
GAPDH, Antibody; Anti-GAPDH Antibody, Rabbit Polyclonal; GAPDH Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-G3PD Antibody; Anti-GAPD Antibody; Anti-HEL-S-162eP Antibody; glyceraldehyde-3-phosphate dehydrogenase; anti-GAPDH antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GAPDH
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GAPDH antibody
IP (Immunoprecipitation), WB (Western Blot)
Conjugation
Unconjugated
Immunogen
Recombinant Human GAPDH Protein;
Preparation
Produced in rabbits immunized with purified, recombinant Human GAPDH (rh GAPDH;P04406; Met1-Glu335). GAPDH specific IgG was purified by Human GAPDH affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(GAPDH was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate Lane B:0.5 mg H1299 Whole Cell Lysate 4 uL anti- GAPDH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- GAPDH rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36kDa Observed band size :36 kDa)
IP (Immunoprecipitation)
(GAPDH was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate Lane B:0.5 mg H1299 Whole Cell Lysate 4 uL anti- GAPDH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- GAPDH rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36kDa Observed band size :36 kDa)
WB (Western Blot)
(Anti-GAPDH rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:36 kDa Observed band size:36 kDa)
WB (Western Blot)
(Anti-GAPDH rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:36 kDa Observed band size:36 kDa)
Related Product Information for anti-GAPDH antibody
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
Product Categories/Family for anti-GAPDH antibody
References
Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 1762 (5): 502-9.Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,053 Da
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 2
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
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Product Notes
The GAPDH gapdh (Catalog #AAA258511) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GAPDH Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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