WB (Western Blot)
(Figure 2. Western blot analysis of GAPDH using anti-GAPDH antibody (AAA126972).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: chicken heart tissue lysates,Lane 2: chicken liver tissue lysates,Lane 3: chicken brain tissue lysates,Lane 4: monkey heart tissue lysates,Lane 5: monkey lung tissue lysates,Lane 6: monkey kidney tissue lysates,Lane 7: monkey liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (#AAA126972) at 0.1ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.)
Rabbit GAPDH Polyclonal Antibody | anti-GAPDH antibody
Anti-GAPDH Antibody Picoband (HRP)
Gene Names
GAPDH; G3PD; GAPD
Reactivity
Chicken, Human, Monkey, Mouse, Rat, Zebrafish
Applications
Western Blot
Purity
HRP-GAPDH antibody is affinity chromatography purified via peptide column.
Synonyms
GAPDH, Antibody; Anti-GAPDH Antibody Picoband (HRP); Glyceraldehyde-3-phosphate dehydrogenase ; HRP-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD; anti-GAPDH antibody
Host
Rabbit
Reactivity
Chicken, Human, Monkey, Mouse, Rat, Zebrafish
Clonality
Polyclonal
Isotype
IgG
Specificity
Widely expressed, with the highest expression in lung, spleen and thymus.
Purity/Purification
HRP-GAPDH antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid. Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
Concentration
0.5mg/mL (varies by lot)
Applicable Applications for anti-GAPDH antibody
WB (Western Blot)
Immunogen
HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
Subcellular Localization
Cytoplasm, cytosol
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Conjugation
HRP
Sequence Similarities
Belongs to the sodium:neurotransmitter symporter (SNF)(TC 2.A.22) family. SLC6A4 subfamily.
Cross Reactivity
HRP-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.
Protein Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Preparation and Storage
HRP-GAPDH antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
WB (Western Blot)
(Figure 2. Western blot analysis of GAPDH using anti-GAPDH antibody (AAA126972).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: chicken heart tissue lysates,Lane 2: chicken liver tissue lysates,Lane 3: chicken brain tissue lysates,Lane 4: monkey heart tissue lysates,Lane 5: monkey lung tissue lysates,Lane 6: monkey kidney tissue lysates,Lane 7: monkey liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (#AAA126972) at 0.1ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.)
WB (Western Blot)
(Figure 2. Western blot analysis of GAPDH using anti-GAPDH antibody (AAA126972).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: chicken heart tissue lysates,Lane 2: chicken liver tissue lysates,Lane 3: chicken brain tissue lysates,Lane 4: monkey heart tissue lysates,Lane 5: monkey lung tissue lysates,Lane 6: monkey kidney tissue lysates,Lane 7: monkey liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (#AAA126972) at 0.1ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (AAA126972).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human A562 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat liver tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (#AAA126972) at 0.1ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (AAA126972).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human A562 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat liver tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (#AAA126972) at 0.1ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.)
Related Product Information for anti-GAPDH antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-GAPDH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,053 Da
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 2
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
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Product Notes
The GAPDH gapdh (Catalog #AAA126972) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GAPDH Antibody Picoband (HRP) reacts with Chicken, Human, Monkey, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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