Rabbit anti-Mouse, Rat GAPDH Polyclonal Antibody | anti-GAPDH antibody

RABBIT ANTI GAPDH (C-TERMINAL)

Cat. Num. AAA49987

• Gene Names: GAPDH; G3PD; GAPD; HEL-S-162eP
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Synonyms: GAPDH, Antibody; RABBIT ANTI GAPDH (C-TERMINAL); anti-GAPDH antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 293

• Applicable Applications for anti-GAPDH antibody: WB (Western Blot)
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3) ; Preparation
• Immunogen: A 16 amino acid peptide located near human GAPDH carboxy-terminus.; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Immunocytochemical staining of HeLa cells with Rabbit anti Human GAPDH (AAA49987))

Application Data

(Western blot analysis of whole cell lysate from HeLa human epithelial adenocarcinoma cells probed with Rabbit anti Human GAPDH (AAA49987) at 0.5(A), 1(B) and 2(C) ug/ml)

Related Product Information for anti-GAPDH antibody

Rabbit anti GAPDH (C-Terminal) antibody recognizes an epitope within the C-Terminal region (CT) of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme which plays a key role in energy production, and has also been implicated in numerous cellular processes. GAPDH is a homotetramer molecule consisting of four 36kDa subunits, constitutively expressed in most cells and tissues, and is responsible for the generation of energy during carbohydrate metabolism, catalysing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate. The role of GAPDH as a multifunctional protein has emerged from many studies, confirming its involvement in critical nuclear pathways, apoptosis, membrane transport and fusion, DNA replication and repair and phosphotransferase activity. Furthermore, the ability of GAPDH to bind with high affinity to beta-amyloid precursor protein (Alzheimers disease) and selectively with CAG mutated proteins, including huntingtin (Huntingdons disease) and the androgen receptor (spinobulbar muscular atrophy), has focused many studies towards the role of GAPDH in the pathogenesis of neurodegenerative diseases (De Santo et al. 2010).

NCBI and Uniprot Product Information

• NCBI GI #: 378404908
• NCBI GeneID: 2597
• NCBI Accession #: NP_001243728.1
• NCBI GenBank Nucleotide #: NM_001256799.2
• UniProt Accession #: P04406; P00354; Q53X65; E7EUT4
• Molecular Weight: 31,548 Da
• NCBI Official Full Name: glyceraldehyde-3-phosphate dehydrogenase isoform 2
• NCBI Official Synonym Full Names: glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Symbol: GAPDH
• NCBI Official Synonym Symbols: G3PD; GAPD; HEL-S-162eP
• NCBI Protein Information: glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; peptidyl-cysteine S-nitrosylase GAPDH
• UniProt Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• UniProt Gene Name: GAPDH
• UniProt Synonym Gene Names: GAPD; GAPDH
• UniProt Entry Name: G3P_HUMAN