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WB (Western Blot)
(Sample (50 ug of whole cell lysate) A: Mouse Brain. 10% SDS PAGE. diluted at 1:10000.)
Rabbit GAPDH Polyclonal Antibody | anti-GAPDH antibody
Rabbit Polyclonal to Human GAPDH
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Drosophila, Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
GAPDH, Antibody; Rabbit Polyclonal to Human GAPDH; Anti-GAPDH Antibody IHC-plus; GAPDH; A1 40 kd subunit; Activator 1 40 kd subunit; G3PD; GAPD; G3pdh; Rfc40; Rf-c 40 kd subunit; Human GAPDH; anti-GAPDH antibody
Host
Rabbit
Reactivity
Drosophila, Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Human GAPDH
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 20% glycerol (pH 7), 0.025% proclin
Concentration
1 mg/ml (varies by lot)
Sequence Length
335
Applicable Applications for anti-GAPDH antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 9 and 323 of GAPDH.
Immunogen Type
Recombinant protein
Immunogen
GAPDH antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 9 and 323 of GAPDH.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
WB (Western Blot)
(Sample (50 ug of whole cell lysate) A: Mouse Brain. 10% SDS PAGE. diluted at 1:10000.)
WB (Western Blot)
(Sample (50 ug of whole cell lysate) A: Mouse Brain. 10% SDS PAGE. diluted at 1:10000.)
WB (Western Blot)
(Sample (30 ug of whole cell lysate). A: 293T. B: A431. C: H1299. D: Hela. 10% SDS PAGE. GAPDH antibody diluted at 1:5000.)
WB (Western Blot)
(Sample (30 ug of whole cell lysate). A: 293T. B: A431. C: H1299. D: Hela. 10% SDS PAGE. GAPDH antibody diluted at 1:5000.)
IF (Immunofluorescence)
(Immunofluorescence of paraformaldehyde-fixed A549, using GAPDH antibody at 1:200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence of paraformaldehyde-fixed A549, using GAPDH antibody at 1:200 dilution.)
IHC (Immunohiostchemistry)
(Anti-GAPDH antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohiostchemistry)
(Anti-GAPDH antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry)
(Anti-GAPDH antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry)
(Anti-GAPDH antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Related Product Information for anti-GAPDH antibody
GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,548 Da
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 1
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD; HEL-S-162eP
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; epididymis secretory sperm binding protein Li 162eP
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
Similar Products
Product Notes
The GAPDH gapdh (Catalog #AAA52255) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human GAPDH reacts with Drosophila, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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