FCM/FACS (Flow Cytometry)
(GAPDHS Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit GAPDHS Polyclonal Antibody | anti-GAPDHS antibody
GAPDHS Antibody (Center)
Gene Names
GAPDHS; GAPD2; GAPDS; HSD-35; GAPDH-2
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GAPDHS, Antibody; GAPDHS Antibody (Center); Glyceraldehyde-3-phosphate dehydrogenase; testis-specific; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; GAPDH-2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; GAPDHS; GAPD2; GAPDH2; GAPDS; anti-GAPDHS antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GAPDHS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-134 amino acids from the Central region of human GAPDHS.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
104-134
Sequence Length
408
Applicable Applications for anti-GAPDHS antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GAPDHS Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GAPDHS Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(GAPDHS Antibody (Center) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GAPDHS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(GAPDHS Antibody (Center) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GAPDHS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of GAPDHS Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). GAPDHS (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of GAPDHS Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). GAPDHS (arrow) was detected using the purified Pab.)
Related Product Information for anti-GAPDHS antibody
GAPDHS is a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.
Product Categories/Family for anti-GAPDHS antibody
References
Welch,J.E., et.al., J. Androl. 21 (2), 328-338 (2000) Goodwin,L.O., et.al., Mol. Hum. Reprod. 6 (2), 127-136 (2000) Benham,F.J. et.al., Genomics 5 (2), 209-214 (1989)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44501
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase, testis-specific
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
NCBI Official Symbol
GAPDHS
NCBI Official Synonym Symbols
GAPD2; GAPDS; HSD-35; GAPDH-2
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase, testis-specific
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific
UniProt Gene Name
GAPDHS
UniProt Synonym Gene Names
GAPD2; GAPDH2; GAPDS; GAPDH-2
UniProt Entry Name
G3PT_HUMAN
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Product Notes
The GAPDHS gapdhs (Catalog #AAA287720) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 104-134. The GAPDHS Antibody (Center) reacts with Human (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDHS can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GAPDHS gapdhs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDHS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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