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FCM/FACS (Flow Cytometry)
(GAS1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse GAS1 Polyclonal Antibody | anti-GAS1 antibody
GAS1 Antibody (N-term)
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GAS1, Antibody; GAS1 Antibody (N-term); Growth arrest-specific protein 1; GAS-1; GAS1; anti-GAS1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-127 amino acids from the N-terminal region of human GAS1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
95-127
Sequence Length
345
Applicable Applications for anti-GAS1 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.
Cellular Location
Cell membrane; Lipid-anchor, GPI- anchor EMBL
Cellular Localization
Cell membrane; Lipid-anchor, GPI- anchor
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GAS1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GAS1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GAS1 Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GAS1 Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(GAS1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAS1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(GAS1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAS1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(GAS1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GAS1 antibody detected the GAS1 protein (arrow).)
WB (Western Blot)
(GAS1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GAS1 antibody detected the GAS1 protein (arrow).)
Related Product Information for anti-GAS1 antibody
Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.
Product Categories/Family for anti-GAS1 antibody
References
Ribeiro, L.A., et al. Am. J. Med. Genet. A 152A (7), 1688-1694 (2010) : Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) : Rittie, L., et al. Aging Cell 8(6):738-751(2009) Zhao, L., et al. J. Biol. Chem. 284(39):26273-26285(2009) Dominguez-Monzon, G., et al. Int. J. Dev. Neurosci. 27(4):305-313(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
growth arrest-specific protein 1
NCBI Official Synonym Full Names
growth arrest-specific 1
NCBI Official Symbol
GAS1
NCBI Protein Information
growth arrest-specific protein 1
UniProt Protein Name
Growth arrest-specific protein 1
UniProt Gene Name
GAS1
UniProt Synonym Gene Names
GAS-1
UniProt Entry Name
GAS1_HUMAN
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Product Notes
The GAS1 gas1 (Catalog #AAA289968) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 95-127. The GAS1 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GAS1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GAS1 gas1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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