IP (Immunoprecipitation)
(GATA3 was immunoprecipitated using:Lane A:0.5 mg Mouse Brain Whole Cell Lysate4 uL anti-GATA3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-GATA3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size :48 kDa)
Rabbit anti-Human GATA3 Polyclonal Antibody | anti-GATA3 antibody
Anti-GATA3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
GATA3, Antibody; Anti-GATA3 Antibody, Rabbit Polyclonal; GATA binding protein 3; Anti-HDR Antibody; Anti-HDRS Antibody; anti-GATA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GATA3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GATA3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human GATA3
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human GATA3, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(GATA3 was immunoprecipitated using:Lane A:0.5 mg Mouse Brain Whole Cell Lysate4 uL anti-GATA3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-GATA3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size :48 kDa)
IP (Immunoprecipitation)
(GATA3 was immunoprecipitated using:Lane A:0.5 mg Mouse Brain Whole Cell Lysate4 uL anti-GATA3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-GATA3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size :48 kDa)
WB (Western Blot)
(Anti-GATA3 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:48 kDa)
WB (Western Blot)
(Anti-GATA3 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:48 kDa)
Related Product Information for anti-GATA3 antibody
GATA3, a member of the GATA zinc finger transcription factor family, and its dysregulation has been implicated in various types of malignancies. GATA3 serves as an oncogene in GC development. The GATA3 transcription factor is one of the most frequently mutated genes in breast cancer. Overexpression of GATA3 in keratinocytes reversed this effect and significantly upregulated filaggrin and, furthermore, filaggrin-2 mRNA expression. GATA3 is involved in the regulation of filaggrin and filaggrin-2 expression during inflammatory conditions in the skin. GATA3 is a transcription factor used clinically as a marker of breast or urothelial differentiation.
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Product Notes
The GATA3 (Catalog #AAA258729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GATA3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GATA3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GATA3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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