Rabbit GATA4 Polyclonal Antibody | anti-GATA4 antibody

Anti-GATA4 Antibody, Rabbit Polyclonal

Cat. Num. AAA254939

• Gene Names: GATA4; ASD2; VSD1
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: GATA4, Antibody; Anti-GATA4 Antibody, Rabbit Polyclonal; GATA4 Antibody, Rabbit PAb, Antigen Affinity Purified; GATA binding protein 4; Anti-ASD2 Antibody; Anti-TACHD Antibody; Anti-TOF Antibody; Anti-VSD1 Antibody; anti-GATA4 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GATA4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-GATA4 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human GATA4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human GATA4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(PGM2 was immunoprecipitated using:Lane A:0.5 mg THP1 Whole Cell Lysate4 uL anti-PGM2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PGM2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 68 kDaObserved band size :70 kDa)

WB (Western Blot)

(Anti-GATA4 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDa)

Related Product Information for anti-GATA4 antibody

GATA4 is a well-known transcription factor of the GATA family implicated in regulation of sex determination and gonadal development in mammals. The transcription factor GATA4 is a critical regulator of cardiac differentiation-specific gene expression. GATA4, a protein related to osteoblast differentiation and mineralization, whose acetylation is essential for cardiac defects. GATA4 gene encoding the GATA binding protein 4, a binding protein that binds to the ANA sequence GATA, increase susceptibility for alcohol use disorder (AUD). The mutations in GATA4 gene induce inherited atrial and ventricular septation defects, which is the most frequent forms of congenital heart defects (CHDs) constituting about half of all cases.

NCBI and Uniprot Product Information

• NCBI GI #: 33188461
• NCBI GeneID: 2626
• NCBI Accession #: NP_002043.2
• NCBI GenBank Nucleotide #: NM_002052.3
• UniProt Accession #: P43694; Q3MJ45; Q5IFM8; B7ZKX0
• Molecular Weight: 44,565 Da
• NCBI Official Full Name: transcription factor GATA-4
• NCBI Official Synonym Full Names: GATA binding protein 4
• NCBI Official Symbol: GATA4
• NCBI Official Synonym Symbols: ASD2; VSD1
• NCBI Protein Information: transcription factor GATA-4; GATA-binding factor 4
• UniProt Protein Name: Transcription factor GATA-4
• UniProt Gene Name: GATA4
• UniProt Entry Name: GATA4_HUMAN