WB (Western Blot)
(Anti-NR6A1 rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: K562 Whole Cell LysateLane D: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:60 kDa(We are unsure as to the identity of these extra bands.))
Rabbit GCNF/NR6A1 Polyclonal Antibody | anti-NR6A1 antibody
Anti-GCNF/NR6A1 Antibody, Rabbit Polyclonal
Gene Names
NR6A1; RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
Reactivity
HumanPredicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
GCNF/NR6A1, Antibody; Anti-GCNF/NR6A1 Antibody, Rabbit Polyclonal; NR6A1 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-GCNF Antibody; Anti-GCNF1 Antibody; Anti-hGCNF Antibody; Anti-hRTR Antibody; Anti-NR61 Antibody; Anti-RTR Antibody; anti-NR6A1 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GCNF/NR6A1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-NR6A1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the center region of the Human NR6A1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human NR6A1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-NR6A1 rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: K562 Whole Cell LysateLane D: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:60 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-NR6A1 rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: K562 Whole Cell LysateLane D: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:60 kDa(We are unsure as to the identity of these extra bands.))
IF (Immunofluorescence)
(Immunofluorescence staining of NR6A1 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NR6A1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of NR6A1 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NR6A1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to nucleus.)
Related Product Information for anti-NR6A1 antibody
NR6A1/CT150, as an orphan receptor, is a novel member of the cancer-testis (CT) antigen family. Nuclear receptor subfamily 6, group A, member 1 (NR6A1) is involved in regulating embryonic stem cell differentiation, reproduction, neuronal differentiation. Down regulation of NR6A1 could reverse epithelial-to-mesenchymal transition (EMT) process in DU145 and PC3 cell lines, and the overexpression could enhance EMT process in 22RV1 cell line. NR6A1 played a prominent role in migration and invasion of PCa cells, and it is indicated that NR6A1 may act as a novel marker for biochemical recurrence after radical prostatectomy.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,908 Da
NCBI Official Full Name
nuclear receptor subfamily 6 group A member 1 isoform 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 6, group A, member 1
NCBI Official Symbol
NR6A1
NCBI Official Synonym Symbols
RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
NCBI Protein Information
nuclear receptor subfamily 6 group A member 1; germ cell nuclear factor variant 1; retinoid receptor-related testis-specific receptor; retinoic acid receptor-related testis-associated receptor
UniProt Protein Name
Nuclear receptor subfamily 6 group A member 1
UniProt Gene Name
NR6A1
UniProt Synonym Gene Names
GCNF; GCNF; hGCNF; RTR; hRTR
UniProt Entry Name
NR6A1_HUMAN
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Product Notes
The NR6A1 nr6a1 (Catalog #AAA254525) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GCNF/NR6A1 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's GCNF/NR6A1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NR6A1 nr6a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCNF/NR6A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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