FCM/FACS (Flow Cytometry)
(GDF15 Antibody (N-term) (AAA289598) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human GDF15 Polyclonal Antibody | anti-GDF15 antibody
GDF15 Antibody (N-term)
Gene Names
GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
GDF15, Antibody; GDF15 Antibody (N-term); Growth/differentiation factor 15, GDF-15, Macrophage inhibitory cytokine 1, MIC-1, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1, Placental TGF-beta, Placental bone morphogenetic protein, Prostate differentiation factor, GDF15, MIC1, PDF, PLAB, PTGFB; anti-GDF15 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GDF15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human GDF15.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
51-79
Applicable Applications for anti-GDF15 antibody
ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Function
Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses (PubMed: , PubMed: 28846097, PubMed: , PubMed: 28846099, PubMed: , PubMed: 29046435). Binds to its receptor, GFRAL, and activates GFRAL expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem (PubMed: , PubMed: 28846097, PubMed: , PubMed: 28846099). It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which contitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions (PubMed: 28953886). On hepatocytes, inhibits growth hormone signaling (By similarity).
Cellular Location
Secreted
Tissue Location
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney (PubMed:9348093) Detected in plasma (at protein level) (PubMed:28572090, PubMed:29046435).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GDF15 Antibody (N-term) (AAA289598) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GDF15 Antibody (N-term) (AAA289598) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(GDF15 Antibody (N-term) (AAA289598) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GDF15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(GDF15 Antibody (N-term) (AAA289598) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GDF15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(GDF15 Antibody (N-term) (AAA289598) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GDF15 antibody detected the GDF15 protein (arrow).)
WB (Western Blot)
(GDF15 Antibody (N-term) (AAA289598) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GDF15 antibody detected the GDF15 protein (arrow).)
Related Product Information for anti-GDF15 antibody
Bone morphogenetic proteins (e.g., BMP9; MIM 605120) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.
Product Categories/Family for anti-GDF15 antibody
References
Anand, I.S., et al. Circulation 122(14):1387-1395(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Staff, A.C., et al. Gynecol. Oncol. 118(3):237-243(2010)
Roth, P., et al. Clin. Cancer Res. 16(15):3851-3859(2010)
Huh, S.J., et al. Am. J. Pathol. 176(6):2948-2957(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Staff, A.C., et al. Gynecol. Oncol. 118(3):237-243(2010)
Roth, P., et al. Clin. Cancer Res. 16(15):3851-3859(2010)
Huh, S.J., et al. Am. J. Pathol. 176(6):2948-2957(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 34140
NCBI Official Full Name
growth/differentiation factor 15
NCBI Official Synonym Full Names
growth differentiation factor 15
NCBI Official Symbol
GDF15
NCBI Official Synonym Symbols
PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
NCBI Protein Information
growth/differentiation factor 15
UniProt Protein Name
Growth/differentiation factor 15
UniProt Gene Name
GDF15
UniProt Synonym Gene Names
MIC1; PDF; PLAB; PTGFB; GDF-15; MIC-1; NAG-1; NRG-1
UniProt Entry Name
GDF15_HUMAN
Similar Products
Product Notes
The GDF15 gdf15 (Catalog #AAA289598) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GDF15 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GDF15 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GDF15 gdf15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GDF15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.