WB (Western Blot)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~23.7 kDa GDNF protein using Rabbit Anti-GDNF Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~23.7 kDa. Other Band(s): 50 kDa is a dimer.)
Rabbit anti-Human, Rat GDNF Polyclonal Antibody | anti-GDNF antibody
GDNF Antibody: ATTO 390
Gene Names
GDNF; ATF; ATF1; ATF2; HSCR3; HFB1-GDNF
Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Peptide Affinity Purified
Synonyms
GDNF, Antibody; GDNF Antibody: ATTO 390; Rabbit Anti-Human GDNF Polyclonal; Glial cell line-derived neurotrophic factor Antibody; Astrocyte-derived trophic factor Antibody; Astrocyte derived trophic factor 1 Antibody; Glial cell derived neurotrophic factor Antibody; ATF 1 Antibody; ATF 2 Antibody; Glial Cell Line Derived Neurotrophic Factor Antibody; ATF Antibody; Atf Antibody; HSCR3 Antibody; Astrocyte derived trophic factor Antibody; ATF2 Antibody; GDNF_HUMAN Antibody; GDNF Antibody; Glial derived neurotrophic factor Antibody; ATF1 Antibody; gdnf Antibody; HFB1 GDNF Antibody; hGDNF Antibody; anti-GDNF antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Specificity
Detects ~24 kDa. In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.
Purity/Purification
Peptide Affinity Purified
Form/Format
PBS, 50% glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
159
Applicable Applications for anti-GDNF antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Synthetic peptide from the mid-protein of Human GDNF
Immunogen Species
Human
Conjugate
ATTO 390
Cellular Localization
Secreted
Certificate of Analysis
A 1:1000 dilution was sufficient for detection of GDNF in 15 ug of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Preparation and Storage
Store at-20 degree C.
WB (Western Blot)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~23.7 kDa GDNF protein using Rabbit Anti-GDNF Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~23.7 kDa. Other Band(s): 50 kDa is a dimer.)
WB (Western Blot)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~23.7 kDa GDNF protein using Rabbit Anti-GDNF Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~23.7 kDa. Other Band(s): 50 kDa is a dimer.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis using Rabbit Anti-GDNF Polyclonal Antibody . Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis using Rabbit Anti-GDNF Polyclonal Antibody . Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X.)
ICC (Immunocytochemistry)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GDNF Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Vesicles, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GDNF Antibody (D) Composite.)
ICC (Immunocytochemistry)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GDNF Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Vesicles, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GDNF Antibody (D) Composite.)
Product Categories/Family for anti-GDNF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
glial cell line-derived neurotrophic factor isoform 5
NCBI Official Synonym Full Names
glial cell derived neurotrophic factor
NCBI Official Symbol
GDNF
NCBI Official Synonym Symbols
ATF; ATF1; ATF2; HSCR3; HFB1-GDNF
NCBI Protein Information
glial cell line-derived neurotrophic factor
UniProt Protein Name
Glial cell line-derived neurotrophic factor
UniProt Gene Name
GDNF
UniProt Synonym Gene Names
hGDNF; ATF
UniProt Entry Name
GDNF_HUMAN
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Product Notes
The GDNF gdnf (Catalog #AAA253953) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GDNF Antibody: ATTO 390 reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GDNF can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GDNF gdnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GDNF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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