WB (Western Blot)
(GFI1B Antibody (C-term) western blot analysis in HL-60,K562,U266cell line and mouse spleen lysates (35ug/lane).This demonstrates the GFI1B antibody detected the GFI1B protein (arrow).)
Rabbit anti-Human, mouse GFI1B Polyclonal Antibody | anti-GFI1B antibody
GFI1B Antibody (C-term)
Gene Names
GFI1B; BDPLT17; ZNF163B
Reactivity
Human, mouse
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GFI1B, Antibody; GFI1B Antibody (C-term); Zinc finger protein Gfi-1b; Growth factor independent protein 1B; Potential regulator of CDKN1A translocated in CML; GFI1B; anti-GFI1B antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GFI1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the C-terminal region of human GFI1B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
302-330
Sequence Length
284
Applicable Applications for anti-GFI1B antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(GFI1B Antibody (C-term) western blot analysis in HL-60,K562,U266cell line and mouse spleen lysates (35ug/lane).This demonstrates the GFI1B antibody detected the GFI1B protein (arrow).)
WB (Western Blot)
(GFI1B Antibody (C-term) western blot analysis in HL-60,K562,U266cell line and mouse spleen lysates (35ug/lane).This demonstrates the GFI1B antibody detected the GFI1B protein (arrow).)
Related Product Information for anti-GFI1B antibody
Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.
Product Categories/Family for anti-GFI1B antibody
References
Roedel B., et al. Genomics 54:580-582(1998).
Huang D.Y., et al. Nucleic Acids Res. 32:3935-3946(2004).
Halleck A., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Humphray S.J., et al. Nature 429:369-374(2004).
Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Huang D.Y., et al. Nucleic Acids Res. 32:3935-3946(2004).
Halleck A., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Humphray S.J., et al. Nature 429:369-374(2004).
Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37492
NCBI Official Full Name
zinc finger protein Gfi-1b isoform 2
NCBI Official Synonym Full Names
growth factor independent 1B transcription repressor
NCBI Official Symbol
GFI1B
NCBI Official Synonym Symbols
BDPLT17; ZNF163B
NCBI Protein Information
zinc finger protein Gfi-1b
UniProt Protein Name
Zinc finger protein Gfi-1b
UniProt Gene Name
GFI1B
UniProt Entry Name
GFI1B_HUMAN
Similar Products
Product Notes
The GFI1B gfi1b (Catalog #AAA289174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 302-330. The GFI1B Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GFI1B can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GFI1B gfi1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFI1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.