WB (Western Blot)
(Detection of human GGA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GGA1 antibody AAA213233 (lot AAA213233-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human GGA1 Polyclonal Antibody | anti-GGA1 antibody
Rabbit anti-GGA1 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
GGA1, Antibody; Rabbit anti-GGA1 Antibody, Affinity Purified; golgi-localized, gamma ear-containing, ARF-binding protein 1; Gamma-adaptin-related protein 1; ADP-ribosylation factor binding protein 1; ADP-ribosylation factor-binding protein GGA1; gamma-adaptin related protein 1; golgi associated, gamma adaptin ear containing, ARF binding protein 1; anti-GGA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-GGA1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 425 and 475
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human GGA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GGA1 antibody AAA213233 (lot AAA213233-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human GGA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GGA1 antibody AAA213233 (lot AAA213233-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human GGA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GGA1 antibody AAA213233 (lot AAA213233-1) used for IP at 6 ug per reaction. GGA1 was also immunoprecipitated by rabbit anti-GGA1 antibody BL21055. For blotting immunoprecipitated GGA1, AAA213233 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human GGA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GGA1 antibody AAA213233 (lot AAA213233-1) used for IP at 6 ug per reaction. GGA1 was also immunoprecipitated by rabbit anti-GGA1 antibody BL21055. For blotting immunoprecipitated GGA1, AAA213233 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-GGA1 antibody
Gamma-adaptin-related protein 1 (GGA1) is a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins [taken from NCBI Entrez Gene (Gene ID: 26088)].
Product Categories/Family for anti-GGA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
72,277 Da
NCBI Official Full Name
ADP-ribosylation factor-binding protein GGA1 isoform 1
NCBI Official Synonym Full Names
golgi-associated, gamma adaptin ear containing, ARF binding protein 1
NCBI Official Symbol
GGA1
NCBI Protein Information
ADP-ribosylation factor-binding protein GGA1
UniProt Protein Name
ADP-ribosylation factor-binding protein GGA1
UniProt Gene Name
GGA1
UniProt Entry Name
GGA1_HUMAN
Similar Products
Product Notes
The GGA1 gga1 (Catalog #AAA213233) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GGA1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GGA1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GGA1 gga1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GGA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.