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WB (Western Blot)
(Anti-GGCT rabbit polyclonal antibody at 1:500 dilutionLane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human GGCT Polyclonal Antibody | anti-GGCT antibody
Anti-GGCT Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
GGCT, Antibody; Anti-GGCT Antibody, Rabbit Polyclonal; GGCT Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-C7orf24 Antibody; Anti-CRF21 Antibody; Anti-GCTG Antibody; Anti-GGC Antibody; gamma-glutamylcyclotransferase; anti-GGCT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GGCT
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. 0.2um filtered solution in PBS
Applicable Applications for anti-GGCT antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human GGCT protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human GGCT (rh GGCT; O75223-1; Met1-Leu188). GGCT specific IgG was purified by Human GGCT affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
WB (Western Blot)
(Anti-GGCT rabbit polyclonal antibody at 1:500 dilutionLane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-GGCT rabbit polyclonal antibody at 1:500 dilutionLane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(GGCT was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate2 uL anti-GGCT rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GGCT rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: 21 kDa)
IP (Immunoprecipitation)
(GGCT was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate2 uL anti-GGCT rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GGCT rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: 21 kDa)
IHC (Immunohistochemisry)
(Immunochemical staining of human GGCT in human corpus uteri with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemisry)
(Immunochemical staining of human GGCT in human corpus uteri with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human GGCT in human hepatoma with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human GGCT in human hepatoma with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human GGCT in human lung cancer with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human GGCT in human lung cancer with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-GGCT antibody
Background: GGCT belongs to the gamma-glutamylcyclotransferase family. It catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism. GGCT may play a significant role in glutathione homeostasis. GGCT also induces release of cytochrome c from mitochondria with resultant induction of apoptosis. Pseudogenes of GGCT gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
References
Wagner SA. et al., 2011, Mol Cell Proteomics. 10 (10): M111.013284. Kim W. et al., 2011, Mol Cell. 44 (2): 325-40. Amano T. et al., 2012, J Histochem Cytochem. 60 (1): 76-86.
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Product Notes
The GGCT (Catalog #AAA257540) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GGCT Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GGCT can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GGCT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GGCT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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