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FCM/FACS (Flow Cytometry)
(GGH Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human GGH Polyclonal Antibody | anti-GGH antibody
GGH Antibody (C-term)
Gene Names
GGH; GH
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GGH, Antibody; GGH Antibody (C-term); Gamma-glutamyl hydrolase; Conjugase; GH; Gamma-Glu-X carboxypeptidase; GGH; anti-GGH antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GGH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 amino acids from the C-terminal region of human GGH.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
229-256
Sequence Length
318
Applicable Applications for anti-GGH antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GGH Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GGH Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GGH Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GGH Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(GGH Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GGH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(GGH Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GGH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(GGH Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GGH antibody detected the GGH protein (arrow).)
WB (Western Blot)
(GGH Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GGH antibody detected the GGH protein (arrow).)
Related Product Information for anti-GGH antibody
This gene catalyzes the hydrolysis of
folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by
the removal of gamma-linked polyglutamates and glutamate. [provided
by RefSeq].
folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by
the removal of gamma-linked polyglutamates and glutamate. [provided
by RefSeq].
Product Categories/Family for anti-GGH antibody
References
Adjei, A.A., et al. J Thorac Oncol 5(9):1346-1353(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Organista-Nava, J., et al. Leuk. Res. 34(6):728-732(2010)
Figueiredo, J.C., et al. Cancer Causes Control 21(4):597-608(2010)
Dervieux, T., et al. Pharmacogenet. Genomics (2009) In press :
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Organista-Nava, J., et al. Leuk. Res. 34(6):728-732(2010)
Figueiredo, J.C., et al. Cancer Causes Control 21(4):597-608(2010)
Dervieux, T., et al. Pharmacogenet. Genomics (2009) In press :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35964
NCBI Official Full Name
gamma-glutamyl hydrolase
NCBI Official Synonym Full Names
gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
NCBI Official Symbol
GGH
NCBI Official Synonym Symbols
GH
NCBI Protein Information
gamma-glutamyl hydrolase
UniProt Protein Name
Gamma-glutamyl hydrolase
UniProt Gene Name
GGH
UniProt Entry Name
GGH_HUMAN
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Product Notes
The GGH ggh (Catalog #AAA285689) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 229-256. The GGH Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GGH can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GGH ggh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GGH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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